Abstract: To know the effect of inoculation methods,substrate and matrix conditions on the incidence of clubroot disease.A stable inoculation technique was developed to inoculate Chinese cabbage with Plasmodiophora brassicae,which could be adoptable in evaluation of resistance of cruciferous to clubroot.The inoculation methods,clubroot storage methods,light sources,photoperiods,substrates and pH environment of culture system etc were studied to compare the affection to disease incidence of clubroot.The results showed that root soaking inoculation,with 7-day-old seedlings cultured on Petri dish,caused more severe and stable symptoms,and was much simple and efficient.Natural light supplemented by artificial light with a 16 h photoperiod was recognized as the best light source for inoculation.Compared with soil and sand,peat and vermiculite were the optimal matrixes for clubroot development.Acid matrixes were favorable for severe attack of the disease,whereas neutral matrixes were less favorable.In addition,the clubroot maintained high pathogenic vitality after air-dried at room temperature and preserved at dry environments,which demonstrated that clubroot drying storage could be recommended as a preservation method in laboratory.

Key words: Clubroot, Plasmodiophora brassicae, Inoculation methods, Photoperiod, Matrixes, Preservation conditions

CLC Number: 

• S636.3