Mapping of Clubroot Resistance Gene and Developing Linkage Markers in Brassica rapa L. ssp. pekinensis

doi:10.7668/hbnxb.201751417

Abstract

Abstract: In order to identify the clubroot resistance gene of Chinese cabbage in G6 and develop linkage markers, high-generation inbred line G4 of Chinese cabbage with high-susceptible to clubroot disease, high-generation inbred line G6 of Chinese cabbage with high-resistant to clubroot disease, F₁ obtained by crossing G4 and G6, and the F₂ generation separation populations constructed by F₁ selfing were used as materials. By artificial inoculation, phenotypic identification and genetic analysis, it was found that clubroot disease resistance in the disease resistance materials were controlled by a dominant single gene. Further expanding the number of F₂ generation populations to map the genes of clubroot disease and screening molecular markers linked to disease resistance genes, the linkage analysis of polymorphic markers was performed using JoinMap 4.0 software, and five InDel markers linked to the resistance gene of Chinese cabbage were obtained. The most recent markers on both sides were BrID10727 and BrID10867, the genetic distance from the disease resistance genes were 4.6,2.5 cM, respectively, and the disease resistance gene was located on the chromosome A08 of Chinese cabbage. In addition, it was found that the newly developed polymorphic marker BrID10381 based on the Crr1 gene sequence was located outside the initial localization range of the newly located clubroot resistance gene, so it could be inferred that the newly located clubroot resistance gene locus was not the same locus as Crr1, and the marker BrID10381 could be used for molecular marker assisted selection of Crr1 gene.

Key words: Chinese cabbage, Clubroot disease, Gene mapping, Linkage marker

CLC Number:

S634.1

WANG Chaonan, ZHANG Bin, ZHANG Hong, WEN Juanjuan, WANG Tao. Mapping of Clubroot Resistance Gene and Developing Linkage Markers in Brassica rapa L. ssp. pekinensis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(3): 203-208. doi: 10.7668/hbnxb.201751417.

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