Development and Validation of Molecular Markers for Resistance to Dry Burning Heart Disease in Chinese Cabbage

doi:10.7668/hbnxb.20192409

Abstract

Abstract: In order to develop closely linked molecular markers for resistance to dry burning heart disease in Qingmaye Chinese cabbage and accelerate the cultivation of high-quality varieties resistant to dry burning heart disease.200 F₂ and 100 BC₁F₁ populations were constructed simultaneously with the excellent Qingmaye high generation inbred line H227 and Baimaye high generation inbred line G83,the resistance and susceptibility phenotypes of the population were identified by in vitro leaf cutting method, and the genetic law of dry burning heart disease in Qingmaye Chinese cabbage was statistically analyzed. At the same time, combined with BSA method, the disease resistance gene of dry burning heart was preliminarily located and labeled by software JoinMap 4.0 and Mapchart.The results showed that the disease grading of F₂ population and BC₁F₁ population constructed by parents with significant difference in resistance and susceptibility to dry burning heart disease showed obvious unimodal distribution, close to normal distribution, and the inheritance law of resistance had the genetic characteristics of quantitative traits.Based on the sequence information of Chinese cabbage genome database and the constructed molecular marker linkage map, the disease resistance gene in the experimental material was located on A07 chromosome, and a molecular marker BrIDCRT07 linked to the dry burning heart disease resistance gene was designed.The genetic distance between the molecular marker and BrID10343 and BrID10349 was 0.13, 0.78 cM respectively.It was verified that the coincidence rate of this marker in F₂ population was 86.8%, and that in BC₁F₁ population was 94.9%. It could be used as an auxiliary screening marker for anti dry burning heart disease resources of Chinese cabbage in the future.

Key words: Chinese cabbage, Dry burning heart disease, Molecular markers, Bulked segregant analysis

CLC Number:

ZHANG Hong, XU Yingli, WANG Chaonan, HUANG Zhiyin, FAN Weiqiang, LI Mei, ZHANG Bin. Development and Validation of Molecular Markers for Resistance to Dry Burning Heart Disease in Chinese Cabbage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(6): 195-203. doi: 10.7668/hbnxb.20192409.

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