Development and Application of Gene Markers in the Exogenous Chromosome Segment of Chinese Cabbage-Cabbage Translation Lines

doi:10.7668/hbnxb.201751153

Abstract

Abstract: In order to identify Chinese cabbage-cabbage translocation line by gene markers, the polymorphism primers were designed according to the nucleotide difference of collinear genes between Chinese cabbage and cabbage, and were screened with Chinese cabbage 85-1 and cabbage 11-1 as test materials. The applicability of the primers was detected using Chinese cabbage-cabbage translocation lines and their self-crossing progenies, Chinese cabbage varieties and cabbage varieties. The results showed that among 171 pairs of primers designed based on 263 collinear-gene sequences of Chinese cabbage and cabbage, 66 pairs of polymorphic primers were confirmed, the ratio being 38.60%. Among the 66 gene markers, two markers from Chinese cabbage gene sequences and 48 markers from cabbage gene sequences could be used as the specific gene markers to distinguish Chinese cabbage and cabbage, which were successfully applied to identify Chinese cabbage-cabbage translocation lines AT1-41 and AT1-47, and the self-crossing progenies of AT1-47. Furthermore, PCR amplification of the 66 gene markers was carried out in four Chinese cabbage varieties and four cabbage varieties, among which 52 markers could distinguished completely the Chinese cabbage varieties and the cabbage varieties, while other 11 markers presented the polymorphism in parts of the varieties. The results established the foundation for further studying the expression, regulation and interaction of exogenous genes in Chinese cabbage-cabbage translocation lines and for marker-assisted breeding.

Key words: Chinese cabbage, Cabbage, Translocation line, Gene marker

CLC Number:

SU Jianhui, ZHANG Leqiang, WANG Ruying, WANG Yanhua, ZHANG Weiwei, MA Cong, XUAN Shuxin, SHEN Shuxing. Development and Application of Gene Markers in the Exogenous Chromosome Segment of Chinese Cabbage-Cabbage Translation Lines[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(3): 89-95. doi: 10.7668/hbnxb.201751153.

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