Structure Characteristics and Prokaryotic Expression Analysis of Soybean Glyma08g11030 Gene

doi:10.7668/hbnxb.201751473

Abstract

Abstract: In order to further clarify the function of Glyma08g11030 gene, sequence analysis and protein domain analysis of Glyma08g11030 were performed by means of bioinformatics and PCR. Bioinformatics analysis showed that the full-length of Glyma08g11030 cDNA was 1 419 bp, with an open reading frame of 1 362 bp, encoding 453 amino acids. The molecular weight of the protein was about 51.288 ku, and the isoelectric point was 8.14. Sequence analysis indicated that the Glyma08g11030 genome contained two exons and one intron. Multiple sequence alignment revealed that the Glyma08g11030 protein contained one F-BOX conserved domain. Phylogenetic tree analysis showed that the protein had a homologous relationship with pigeonpea, kidney bean, peanut, cowpea and adzuki bean. In order to obtain the purified Glyma08g11030 protein, the amplified fragment was cloned into the prokaryotic expression vector pET28a, and the recombinant plasmid pET28a-Glyma08g11030 was constructed. The target protein was obtained by SDS-PAGE. The results showed that the protein mainly existed in the form of inclusion bodies. The results of this study laid the foundation for further purification and identification of Glyma08g11030 protein and investigation of its function.

Key words: Soybean, Glyma08g11030, Prokaryotic expression

CLC Number:

S565.1

WANG Ping, YU Yuehua, BAI Yucui, WAN Huina, NI Zhiyong. Structure Characteristics and Prokaryotic Expression Analysis of Soybean Glyma08g11030 Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(3): 82-88. doi: 10.7668/hbnxb.201751473.

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