Identification of the Bacterial Soft Rot Pathogens on Chinese Cabbage in Beijing

doi:10.7668/hbnxb.2018.03.011

Abstract

Abstract: To identify the pathogens causing bacterial soft rot on Chinese cabbage in Beijng,29 pathogenic strains were isolated and cultured from diseased samples,which were collected from several districts in Beijing. Morphology analyses,physiological and biochemical tests,molecular assay and pathogenicity test were performed to elucidate the causative bacterial pathogens. The results showed that the 17 strains were identified as Pectobacterium carotovorum subsp.brasiliensis (Pcb),and the remaining 12 strains were identified as P. carotovorum subsp. carotovorum (Pcc). Moreover,there were 9 atypical Pcc strains,which were unable to degrade citrate,or failed to grow at 37℃ and 7% NaCl,or weakly utilized palatinose,D-maltose and D-arabitol. The results of the pathogenicity analysis showed that all the strains expressed high pathogenicity with the exception of 2 Pcb strains and 1 Pcc strain exhibiting medium pathogenicity. The bacterial soft rot disease of Brassica rapa subsp. pekinensis occurred in Beijing and was caused mainly by Pcb and Pcc with high virulence,which will provide the basis for the disease control.

Key words: Chinese cabbage, Bacterial soft rot, Pathogen identification, Pectobacterium carotovorum

CLC Number:

S432.1

LI Xiaoying, TIAN Yu, ZHAO Liang, CHEN Changlong, XIE Hua. Identification of the Bacterial Soft Rot Pathogens on Chinese Cabbage in Beijing[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(3): 63-70. doi: 10.7668/hbnxb.2018.03.011.

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