Chinese Cabbage Root Disease Genetic Law and Positioning of Resistance Genes Research

doi:10.7668/hbnxb.2017.04.010

Abstract

Abstract: To explore the root disease resistance genetic regularity,by root disease resistance difference material,G57 and G70,the F₂ segregation population containing 500 per plant was built.Through artificial inoculation of the parents,F₁ and F₂ segregation population phenotypic characterization results were statistically analyzed. The results showed that root disease resistance of materials was controlled by a single dominant gene. In order to further localization of resistance genes in test materials,we used 678 molecular markers of parents,F₁ and F₂ population screening verification,resistance genes was preliminarily located in molecular marker KBRH129J18 and TCR02-F.This test was based on two chain tags,chain development design closer chain of molecular markers,ultimately with resistance genes chain 5 of SSR molecular markers,respectively,Bra0345-1,Bra0235-2,Bra0235-1,Bra19317,Bra019392. Their genetic distance with resistance genes were 2.4,2.4,2.4,2.5,3.3 cM.Validated,development and design of polymorphism markers in Chinese cabbage had good applicability.

Key words: Chinese cabbage, Root disease, Disease resistance genetic law, Resistance genes, Molecular markers

CLC Number:

S634.1

ZHANG Hong, ZHANG Bin, WEN Fengying, LIU Xiaohui, WANG Chaonan, LI Mei, HUANG Zhiyin. Chinese Cabbage Root Disease Genetic Law and Positioning of Resistance Genes Research[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(4): 60-66. doi: 10.7668/hbnxb.2017.04.010.

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