QTL Analysis of Gummy Stem Blight in Melon by SALF-seq and BSA Technique

doi:10.7668/hbnxb.20191702

Abstract

Abstract: In order to investigate the resistant genes location of gummy stem blight(GSB) on chromosomes of melon, the resistance parent P181, susceptible parent P01 and F₂ progeny were used for GSB resistance genes identification. According to the disease incidence of GSB of F₂,two parents, 30 high-resistance and 30 high-suspect F₂ generations were choice to isolated DNA, in order to construct resistant and susceptible pools. The QTL locations were applicated by SLAF-seq and BSA technique. A total of 188 022 SLAF-labels were obtained, after low-quality SNP sites filterted out, 5 676 high-quality SNP markers were developed. Two GSB-resistance QTLs were identified on chromosomes of melon by the methods of SNP-index, which were located in Chr01 and Chr04, with 1.19 Mb (on the region of 9 837 447-11 028 838 bp) and 0.86 Mb (on the region of 33 135 224-33 993 540 bp) interval, respectively. A total of 218 genes were analysis in QTLs. Furthermore, according to the gene function annotations, 15 candidate genes were speculated with the relationship of GSB resistance, including 1 gene of NBS-LRR, 5 genes of E3 ubiquitin-protein ligase, 1 gene of RPM1-interacting protein, 1 gene of zinc finger protein, 1 gene of NAC domain-containing protein, 2 genes of calcium-dependent protein kinase, 3 genes of L-type lectin-domain containing receptor kinase, 1 gene of RING-H2 finger protein. The results will support reference of the basis of resistance genes of GSB.

Key words: Melon, Gummy stem blight, Resistance genes, SLAF-seq, QTL

CLC Number:

Q78
S652.03

GAO Tianyi, HAO Fangmin, ZANG Quanyu, MA Erlei, HUANG Yunping, WANG Yuhong. QTL Analysis of Gummy Stem Blight in Melon by SALF-seq and BSA Technique[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(2): 40-45. doi: 10.7668/hbnxb.20191702.

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