Prokaryotic Expression of Transcription Factor StTCP13 in Potato and Its Function of Salt Stress

doi:10.7668/hbnxb.20191507

Abstract

Abstract: In order to investigate the function of StTCP13 in salt stress and lay the foundation for the functional study of StTCP13 in response to abiotic stress of potato. The coding sequence of StTCP13 was obtained by homologous cloning. The protein domain and phylogenetic tree were analyzed by bioinformatics.The results showed that the StTCP13 gene was cloned from the potato variety Favorita.The coding sequence length of StTCP13 was 669 bp, encoding a protein composed of 222 amino acid residues.The results of prokaryotic expression suggested that the molecular weight of fusion protein was 51 ku.Protein domain analysis suggested that the protein contained a conserved TCP domain.The results of phylogenetic tree revealed that the protein had a high homology with the TCP13 in Solanum pennellii and Capsicum annuum.Expression pattern of StTCP13 was analyzed by qRT-PCR.Expression analysis showed that the StTCP13 exhibited different expression levels in root, stem, leaf, bud and bud eye in potato. The StTCP13 had high expression level in root, low expression level in bud eye, in addition,the FPKM of StTCP13 in Spud DB showed that the expression of StTCP13 was induced by high-salinity, drought and ABA treatments.The StTCP13-pGEX6P1 prokaryotic expression vector was constructed by double enzyme digestion. Prokaryotic expression results showed that the StTCP13-GST tag fusion protein was successfully expressed in Escherichia coli BL21 . Salt stress phenotype analysis suggested that StTCP13 could improve the tolerance to salt stress in E.coli BL21 . Above all, the StTCP13 might play a role in response to salt stress in potato.

Key words: Potato, Salt stress, Phenotypic analysis, TCP, Expression pattern, Prokaryotic expression, Transcription factor

CLC Number:

Q78
S532.03

LI Jiahao, XIE Minqiu, WAN Chuanyin, ZUO Ruijie, LU Liming, LI Liqin. Prokaryotic Expression of Transcription Factor StTCP13 in Potato and Its Function of Salt Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(2): 33-39. doi: 10.7668/hbnxb.20191507.

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