Genetic Analysis and QTL Mapping of Plant Height-related Characters in Barley

doi:10.7668/hbnxb.20191631

Abstract

Abstract: In order to discover the QTL of barley plant height-related characters, improve the barley plant height-related characters. Chinese forage barley Taixing 9425 and Japanese beer barley Naso Nijo as well as 177 doubled haploid lines, generated from a cross between Taixing 9425 and Naso Nijo, were used as materials to examine the three plant traits of plant height(PH), internode length under spike(SIL), and spike length(SL) in two environments. Characters, combined with the constructed molecular marker linkage map, using Windows QTL IciMaping V4.1.0.0 software based on the complete interval mapping method for QTL mapping analysis, also the genetic characteristics were analyzed. The results showed that:plant height, spike length, and internode length under spike were mainly controlled by genetic factors, and at the same time affected by the ecological conditions and production conditions of the pilot. The heritability of the three traits was 78.98%, 89.31% and 84.50%, respectively; Plant height was extremely significant positive correlation with the spike length and the internode length under the spike. Plant height and spike length, plant height and the internode length under the spike, and spike length and the internode length under the spike were all significantly positively correlated, with correlation coefficients of 0.688, 0.862 and 0.600, respectively, indicating that the higher the plant height, the longer the spike length and the internode length under the spike. The 2 pilot sites mapped 6 QTL for plant height, 5 QTL for spike length, and 4 QTL for internode length under spike. A total of 15 QTL are located on all chromosomes except chromosomes 1 and 6. The LOD value was 3.50-32.46, the explanation rate of phenotypic variation was 1.53%-38.30%. Among them, qPH-4-1, qPH-7-2, qSL-2-1, qSL-2-2, qSIL-2-1 and qSIL-3-2 have not been reported, and may be new loci. This lay the foundation for the improvement of barley plant height-related characters and molecular marker assisted breeding.

Key words: Barley, Height-related characters, QTL mapping, Double haploid line population, Genetic analysis

CLC Number:

S512.03
Q78

YAO Jiayan, YU Guoqi, HONG Yi, Lü Chao, XU Rugen. Genetic Analysis and QTL Mapping of Plant Height-related Characters in Barley[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(2): 28-32. doi: 10.7668/hbnxb.20191631.

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