Morphological Indexes and QTL Analysis of Stem Lodging Resistance in Wheat

doi:10.7668/hbnxb.20193602

Abstract

Abstract:

In order to clarify the relationship between the morphological and structural characteristics of stem basal node 2 and lodging resistance in wheat,we explored key stem morphological indicators and Quantitative trait loci(QTL)sites for lodging resistance.120 RILs families were selected as research materials,and stem strength,basal second internode length,stem diameter,wall thickness,cellulose content and lignin content were measured in 2020 and 2021,respectively.Multiple regression analysis and QTL locations were performed by combining 55K SNP data.The results showed that the stem strength was significantly or extremely significantly positively correlated with the stem diameter and wall thickness of the second basal internode,and was extremely significantly positively correlated with the cellulose content and lignin content of the second basal internode.Multiple regression analysis showed that cellulose content in basal second internode was the key index affecting stalk strength of wheat.A total of 19 QTLs related to stem traits were detected on chromosomes 1A,1D,2B,2D,4D,5A,5B,5D and 7B,explaining 7.67% to 65.33% of the phenotypic variation.On chromosome 1D,the QTL linked to AX-110771095 and AX-109431570 simultaneously controlled the basal second internode length,wall thickness and cellulose content,explaining the phenotypic contribution of 7.96%-10.76%.

Key words: Wheat, Stalk strength, QTL mapping, Lodging, Multiple regression analysis

WANG Tao, ZHANG Yi, ZHAO Xiaoxue, CHEN Can, SI Hongqi, MA Chuanxi, LU Jie. Morphological Indexes and QTL Analysis of Stem Lodging Resistance in Wheat[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(2): 99-105. doi: 10.7668/hbnxb.20193602.

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References 32

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性状 Trait	环境 Environment	安农0711 Annong 0711	河农825 Henong 825	最小值 Minimum	最大值 Maximum	平均值±标准差 Mean±s	变异系数/% CV	偏度 Skewness	峰度 Kurtness
SS	20HF	1.53	0.99	0.60	3.83	1.61±0.62	38	0.48	0.68
	20HB	1.81	0.93	0.68	2.23	1.36±0.35	26	-0.44	0.26
	21HB	1.75	1.15	0.43	2.38	1.39±0.42	30	-0.35	0.18
	21JY	2.13	1.31	0.80	2.82	1.63±0.50	31	-0.88	0.34
SBL	20HF	68.80	76.88	57.00	112.75	78.45±10.93	14	0.37	0.69
	20HB	68.81	78.56	58.00	114.25	76.79±10.78	14	0.37	0.64
	21HB	60.17	66.95	41.40	99.99	63.02±10.26	16	0.90	0.63
	21JY	68.43	75.70	52.88	103.08	74.53±10.61	14	0.10	0.58
SBD	20HF	4.44	3.80	3.23	5.48	4.40±0.49	11	-0.58	-0.10
	20HB	4.69	4.10	2.38	5.30	4.35±0.50	12	0.72	-0.62
	21HB	4.89	4.35	3.44	5.29	4.50±0.38	8	-0.11	-0.41
	21JY	5.11	4.28	3.29	5.23	4.52±0.41	9	-0.14	-0.59
SWT	20HF	0.57	0.45	0.30	1.04	0.54±0.12	23	1.71	0.74
	20HB	0.55	0.41	0.29	0.67	0.52±0.07	13	1.24	-0.74
	21HB	1.08	0.82	0.69	1.20	0.93±0.10	10	0.22	0.03
	21JY	1.04	0.85	0.70	1.20	0.92±0.10	10	0.10	-0.03
SCC	20HF	26.36	17.99	14.15	35.59	23.67±3.90	16	0.78	0.43
	20HB	21.19	17.64	16.57	38.34	25.20±3.52	14	1.14	0.39
SLC	20HF	20.73	15.34	15.20	25.07	21.07±2.25	11	-0.34	-0.47
	20HB	22.26	16.16	15.41	24.80	20.36±2.36	12	-0.92	0.21

性状 Trait	环境 Environment	安农0711 Annong 0711	河农825 Henong 825	最小值 Minimum	最大值 Maximum	平均值±标准差 Mean±s	变异系数/% CV	偏度 Skewness	峰度 Kurtness
SS	20HF	1.53	0.99	0.60	3.83	1.61±0.62	38	0.48	0.68
	20HB	1.81	0.93	0.68	2.23	1.36±0.35	26	-0.44	0.26
	21HB	1.75	1.15	0.43	2.38	1.39±0.42	30	-0.35	0.18
	21JY	2.13	1.31	0.80	2.82	1.63±0.50	31	-0.88	0.34
SBL	20HF	68.80	76.88	57.00	112.75	78.45±10.93	14	0.37	0.69
	20HB	68.81	78.56	58.00	114.25	76.79±10.78	14	0.37	0.64
	21HB	60.17	66.95	41.40	99.99	63.02±10.26	16	0.90	0.63
	21JY	68.43	75.70	52.88	103.08	74.53±10.61	14	0.10	0.58
SBD	20HF	4.44	3.80	3.23	5.48	4.40±0.49	11	-0.58	-0.10
	20HB	4.69	4.10	2.38	5.30	4.35±0.50	12	0.72	-0.62
	21HB	4.89	4.35	3.44	5.29	4.50±0.38	8	-0.11	-0.41
	21JY	5.11	4.28	3.29	5.23	4.52±0.41	9	-0.14	-0.59
SWT	20HF	0.57	0.45	0.30	1.04	0.54±0.12	23	1.71	0.74
	20HB	0.55	0.41	0.29	0.67	0.52±0.07	13	1.24	-0.74
	21HB	1.08	0.82	0.69	1.20	0.93±0.10	10	0.22	0.03
	21JY	1.04	0.85	0.70	1.20	0.92±0.10	10	0.10	-0.03
SCC	20HF	26.36	17.99	14.15	35.59	23.67±3.90	16	0.78	0.43
	20HB	21.19	17.64	16.57	38.34	25.20±3.52	14	1.14	0.39
SLC	20HF	20.73	15.34	15.20	25.07	21.07±2.25	11	-0.34	-0.47
	20HB	22.26	16.16	15.41	24.80	20.36±2.36	12	-0.92	0.21

性状 Traits	SS
性状 Traits	20HF	20HB	21HB	21JY
SBL	-0.04	-0.11	-0.28^**	-0.28^**
SBD	0.21^*	0.18^*	0.21^*	0.31^**
SWT	0.44^**	0.19^*	0.43^**	0.28^**
SCC	0.50^**	0.49^**
SLC	0.60^**	0.28^**

性状 Traits	SS
性状 Traits	20HF	20HB	21HB	21JY
SBL	-0.04	-0.11	-0.28^**	-0.28^**
SBD	0.21^*	0.18^*	0.21^*	0.31^**
SWT	0.44^**	0.19^*	0.43^**	0.28^**
SCC	0.50^**	0.49^**
SLC	0.60^**	0.28^**

性状 Traits	系数 Coefficient	标准误 Standard error	显著性 Significance	方差膨胀系数 VIF	拟合优度 R²
常量	-1.96	0.42	0		0.41
SLC	0.11	0.03	0	1.60
SCC	0.03	0.01	0.01	1.48
SWT	0.86	0.41	0.04	1.33

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