Expression Analysis of 10 ZmDOFs under Abiotic Stress in Maize

doi:10.7668/hbnxb.20191701

Abstract

Abstract: In order to study the role and function of DNA-binding with one finger (DOF) proteins in maize growth and development, the expression profiles of 10 ZmDOFs genes in different tissues or in response to various abiotic stresses have been carefully studied using qRT-PCR. The result of the phylogenetic tree and gene structure analysis showed that 10 ZmDOFs divided into three subclasses. The result of qRT-PCR revealed that 10 ZmDOFs exhibited obvious expression preference or specificity in different organs, 7 ZmDOFs high expression in tassels, 2 ZmDOFs in young embryo after pollination 15 days and 1 ZmDOF in root, indicating the important roles in the processes of maize growth and development. Results by qRT-PCR revealed the altered expression pattern of most of ZmDOFs in response to salt or osmotic stresses, indicating these gene involved in the regulation of salt or osmotic stress response pathway. ZmDOF3 was up-regulated by at least 5-fold under NH₄⁺ depletion treatment, while ZmDOF23 and ZmDOF46 reduced by 90% under NO₃^- depletion treatment, indicated these genes were involved in nitrogen homeostasis or signaling pathway.

Key words: Maize, DOF transcription factor, Abiotic stress, Phylogenetic tree, Tissue expression

CLC Number:

S513.03
Q78

JIA Liqiang, ZHAO Qiufang, CHEN Shu. Expression Analysis of 10 ZmDOFs under Abiotic Stress in Maize[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(2): 19-27. doi: 10.7668/hbnxb.20191701.

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References

[1] Yanagisawa S. Dof domain proteins:plant-specific transcription factors associated with diverse phenomena unique to plants[J]. Plant Cell Physiol,2004,45(4):386-391. doi:10.1093/pcp/pch055.
[2] Yanagisawa S, Izui K. Molecular cloning of two DNA-binding proteins of maize that are structurally different but interact with the same sequence motif[J]. J Biol Chem,1993,268(21):16028-16036. doi:10.1016/S0021-9258(18) 82353-5.
[3] Yang G Y, Gao X Q, Ma K H, Li D P, Jia C X, Zhai M Z, Xu Z G. The walnut transcription factor JrGRAS2 contributes to high temperature stress tolerance involving in Dof transcriptional regulation and HSP protein expression[J]. BMC Plant Biology, 2018,18(1):367-370. doi:10.1186/s12870-018-1568-y.
[4] Qin H, Wang J, Chen X B, Wang F F, Peng P, Zhou Y, Miao Y C, Zhang Y Q, Gao Y D, Qi Y D, Zhou J H, Huang R F. Rice OsDOF15 contributes to ethylene-inhibited primary root elongation under salt stress[J]. The New Phytologist, 2019,223(2):798-813. doi:10.1111/nph.15824.
[5] Zhou Y, Cheng Y, Wan C P, Li J W, Yang Y X, Chen J J. Genome-wide characterization and expression analysis of the Dof gene family related to abiotic stress in watermelon[J]. Peer J, 2020,8:e8358. doi:10.7717/peerj.8358.
[6] Liu Y, Liu N N, Deng X, Liu D M, Li M F, Cui D D, Hu Y K, Yan Y M. Genome wide analysis of wheat DNA-binding with one finger (Dof) transcription factor genes:evolutionary characteristics and diverse abiotic stress responses[J]. BMC Genomics,2020,21(1):276-289. doi:10.1186/s12864-020-6691-0.
[7] Liu J, Cheng Z C, Xie L H, Li X Y, Gao J. Multifaceted role of Phedof12-1 in the regulation of flowering time and abiotic stress responses in Moso bamboo (Phyllostachys edulis)[J]. International Journal of Molecular Sciences,2019,20(2):424-430. doi:10.3390/ijms20020424.
[8] Ruta V, Longo C, Lepri A, De Angelis V, Occhigrossi S, Costantino P, Vittorioso P. The DOF transcription factors in seed and seedling development[J]. Plants,2019,9(2):218-228. doi:10.3390/plants9020218.
[9] Zou Z, Zhang X C. Genome-wide identification and comparative evolutionary analysis of the Dof transcription factor family in physic nut and castor bean[J]. Peer J,2019,7(17):e6354. doi:10.7717/peerj.6354.
[10] Washio K. Identification of Dof proteins with implication in the gibberellin-regulated expression of a peptidase gene follo wing the germination of rice grains[J]. Biochim Biophys Acta, 2001,1520(1):54-62. doi:10.1016/s0167-4781(01) 00251-2.
[11] Mena M, Cejudo F J, Isabel-Lamoneda I, Carbonero P. A role for the DOF transcription factor BPBF in the regulation of gibberellin-responsive genes in barley aleurone[J]. Plant Physiol,2002,130(1):111-119.doi:10.1104/pp.005561.
[12] Khaksar G, Sangchay W, Pinsorn P, Sangpong L, Sirikantaramas S. Genome-wide analysis of the Dof gene family in durian reveals fruit ripening-associated and cultivar-dependent Dof transcription factors[J]. Scientific Reports,2019,9(1):12109-12111. doi:10.1038/s41598-019-48601-7.
[13] Plesch G, Ehrhardt T, Mueller-Roeber B. Involvement of TAAAG elements suggests a role for Dof transcription factors in guard cell-specific gene expression[J]. The Plant Journal,2001,28(4):455-464.doi:10.1046/j.1365-313x.2001.01166.x.
[14] Negi J, Moriwaki K, Konishi M, Yokoyama R, Nakano T, Kusumi K, Hashimoto-Sugimoto M, Schroeder J I, Nishitani K, Yanagisawa S, Iba K. A Dof transcription factor, SCAP1, is essential for the development of functional stomata in Arabidopsis[J]. Curr Biol,2013,23(6):479-484. doi:10.1016/j.cub.2013.02.001.
[15] Papi M, Sabatini S, Altamura M M, Hennig L, Schäfer E, Costantino P, Vittorioso P. Inactivation of the phloem-specific Dof zinc finger gene DAG1 affects response to light and integrity of the testa of Arabidopsis seeds[J]. Plant Physiol,2002,128(2):411-417. doi:10.1104/pp.010488.
[16] Gualberti G, Papi M, Bellucci L, Ricci I, Bouchez D, Camilleri C, Costantino P, Vittorioso P. Mutations in the Dof zinc finger genes DAG2 and DAG1 influence with opposite effects the germination of Arabidopsis seeds[J]. The Plant Cell,2002,14(6):1253-1263. doi:10.1105/tpc.010491.
[17] Huang Y, Han Z M, Cheng N N, Luo M F, Bai X F, Xing Y Z. Minor effects of 11 Dof family genes contribute to the missing heritability of heading date in rice (Oryza sativa L.)[J]. Frontiers in Plant Science,2020,10:1739.doi:10.3389/fpls.2019.01739.
[18] Skirycz A, Radziejwoski A, Busch W, Hannah M A, Czeszejko J, Kwaśniewski M, Zanor M, Lohmann J U, De Veylder L, Witt I, Mueller-Roeber B. The DOF transcription factor OBP1 is involved in cell cycle regulation in Arabidopsis thaliana[J]. Plant J,2008,56(5):779-792. doi:10.1111/j.1365-313X.2008.03641.x.
[19] Gupta S, Malviya N, Kushwaha H, Nasim J, Bisht N C, Singh V K, Yadav D. Insights into structural and functional diversity of Dof (DNA binding with one finger) transcription factor[J]. Planta,2015,241(3):549-562. doi:10.1007/s00425-014-2239-3.
[20] Moreno-Risueno M A, Martínez M, Vicente-Carbajosa J, Carbonero P. The family of DOF transcription factors:from green unicellular algae to vascular plants[J]. Mol Genet Genomics, 2007,277(4):379-390. doi:10.1007/s00438-006-0186-9.
[21] Lijavetzky D, Carbonero P, Vicente-Carbajosa J. Genome-wide comparative phylogenetic analysis of the rice and Arabidopsis Dof gene families[J]. BMC Evol Biol,2003,23(3):7-13. doi:10.1186/1471-2148-3-17.
[22] Guo Y, Qiu L J. Genome-wide analysis of the Dof transcription factor gene family reveals soybean-specific duplicable and functional characteristics[J]. PLoS One,2013,8(9):e76809.doi:10.1371/journal.pone.0076809.
[23] Cai X F, Zhang Y Y, Zhang C J, Zhang T Y, Hu T X, Ye J, Zhang J H, Wang T T, Li H X, Ye Z B. Genome-wide analysis of plant-specific Dof transcription factor family in tomato[J]. J Integr Plant Biol, 2013, 55(6):552-566. doi:10.1111/jipb.12043.
[24] Chen Y Z, Cao J. Comparative analysis of dof transcription factor family in maize[J]. Plant Mol Biol Rep, 2015,33(5):1245-1258. doi:10.1007/s11105-014-0835-9.
[25] Zou Z, Zhu J L, Zhang X C. Genome-wide identification and characterization of the Dof gene family in cassava (Manihot esculenta)[J]. Gene, 2019,687:298-307. doi:10.1016/j.gene.2018.11.053.
[26] Chen X Y, Wang D X, Liu C, Wang M Z, Wang T, Zhao Q, Yu J J. Maize transcription factor Zmdof1 involves in the regulation of Zm401 gene[J]. Plant Growth Regulation, 2012,66(3):271-284. doi:10.1007/s10725-011-9651-5.
[27] Kumar R, Taware R, Gaur V S, Guru S K, Kumar A. Influence of nitrogen on the expression of TaDof1 transcription factor in wheat and its relationship with photosynthetic and ammonium assimilating efficiency[J]. Molecular Biology Reports,2009,36(8):209-221. doi:10.1007/s11033-008-9436-8.
[28] Wu Y F, Yang W Z, Wei J H, Yoon H, An G. Transcription factor OsDOF18 controls ammonium uptake by inducing ammonium transporters in rice roots[J]. Molecules and Cells, 2017,40(3):178-185. doi:10.14348/molcells.2017.2261.
[29] Jue D J, Sang X L, Lu S Q, Dong C, Zhao Q F, Chen H L, Jia L Q. Genome-wide identification, phylogenetic and expression analyses of the Ubiquitin-conjugating enzyme gene family in maize[J]. PLoS One, 2015,10(11):e0143488.doi:10.1371/journal.pone.0143488.
[30] da Silva D C, da Silveira Falavigna V, Fasoli M, Buffon V, Porto D D, Pappas G J, Pezzotti M, Pasquali G, Revers L F. Transcriptome analyses of the Dof-like gene family in grapevine reveal its involvement in berry, flower and seed development[J]. Horticulture Research,2016,3:16042. doi:10.1038/hortres.2016.42.
[31] Corrales A R, Carrillo L, Lasierra P, Nebauer S G, Dominguez-Figueroa J, Renau-Morata B, Pollmann S, Granell A, Molina R V, Vicente-Carbajosa J, Medina J. Multifaceted role of cycling DOF factor 3(CDF3) in the regulation of flowering time and abiotic stress responses in Arabidopsis[J]. Plant Cell and Enviroment, 2017,40(5):748-764. doi:10.1111/pce.12894.
[32] Corrales A R, Nebauer S G, Carrillo L, Fernández-Nohales P, Marqués J, Renau-Morata B, Granell A, Pollmann S, Vicente-Carbajosa J, Molina R V, Joaquín M. Characterization of tomato cycling dof factors reveals conserved and new functions in the control of flowering time and abiotic stress responses[J]. Journal of Experimental Botany,2014,65(4):995-1012. doi:10.1093/jxb/ert451.
[33] Peña P A, Quach T, Sato S, Ge Z X, Nersesian N, Changa T, Dweikat I, Soundararajan M, Clemente T E. Expression of the maize dof1 transcription factor in wheat and Sorghum[J]. Frontiers in Plant Science,2017,8:434-439. doi:10.3389/fpls.2017.00434.

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