Cloning of SlUDP Gene and Its Response to Cadmium,Drought and Salt Stress in Lycopersicon esculentum Mill.

doi:10.7668/hbnxb.20191459

Abstract

Abstract: Abiotic stress environment severely restricted tomato production, so it is particularly important to find genes related to abiotic stress tolerance in tomato.In the early stage of this study, UDP-glycyltransferase gene screened by high-throughput sequencing was able to participate in the response to cadmium stress in tomatoes. Therefore, this study took tomatoes as the test material to further study the anti-resistance function of this gene.First, the full length UDP cDNA sequence was obtained by homologous cloning method, and the sequence was analyzed by bioinformatics.Phylogenetic tree was used to analyze the phylogenetic relationship between the protein and plants.Secondly, Real-time fluorescence quantitative PCR was used to analyze the tissue expression characteristics of the gene and its expression patterns under three different abiotic stress conditions (Cd, PEG-6000, NaCl).The stress phenotype of transgenic yeast was further analyzed.The sequencing results showed that the cDNA of this gene was 1 486 bp in length, containing a 1 452 bp open reading frame (ORF), encoding 483 amino acids, and was named SlUDP.Phylogenetic tree analysis showed that the protein was most closely related to potato.The results of Real-time fluorescence quantitative PCR showed that the expression of this gene was different in different tissues of tomato, and the expression level was the highest in leaves, and the expression level in each part was root > fruit > lateral stem > main stem > flower.The stress phenotype analysis results of transgenic yeast showed that SlUDP gene improved the yeast's tolerance to Cd and drought.According to the above results, SlUDP may play a certain role in tomato response to abiotic stresses such as heavy metals cadmium, high salt and drought.

Key words: Tomato, SlUDP gene, Abiotic stress, Expression analysis, Functional analysis

CLC Number:

Q78
S641.03

GENG Xinxin, YU Lijie, CHEN Chao, JIN Xiaoxia. Cloning of SlUDP Gene and Its Response to Cadmium,Drought and Salt Stress in Lycopersicon esculentum Mill.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(2): 46-53. doi: 10.7668/hbnxb.20191459.

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