Abstract: In order to explore the effect of CO₂ on SOD, POD and CAT activity and their gene expressions under high temperature, the grafting cucumber in greenhouse was used as material, trying to study the changes of cucumber leaf SOD, POD and CAT activity and gene expression under high temperature after treating with CO₂.The results showed SOD,POD and CAT activity in cucumber leaf were increased after treating with CO₂ under high temperature. Comparing with room temperature treatment,the max difference of cucumber SOD,POD and CAT activity were at 42,28 and 21 days after treating with CO₂ and high temperature,and increased by 19.1%,50.4% and 45.0% respectively. Using Real-time PCR analysis cucumber SOD, POD and CAT gene expression were all increased after treating with CO₂ under high temperature. The max difference of cucumber SOD, POD and CAT gene expression level were at 28,28 and 21 days after treating with CO₂ and high temperature,and with 60.7%,70.3% and 44.9% higher than room temperature treatment respectively. In conclusion,after treating with CO₂,the cucumber SOD,POD and CAT activity and their gene expression level were both increased under high temperature.

Key words: Cucumber, High temperature, CO₂, Antioxidase, Gene expression

CLC Number: 

• S642.2