Identification and Expression Analysis of NLP Transcription Factor Family of Chenopodium quinoa Willd.

doi:10.7668/hbnxb.20191913

Abstract

Abstract: NLP (Nin-Like Protein) transcription factors belong to a plant-specific transcription factors family, which play an important role in plant nitrogen uptake, transport and assimilation. To understand the whole genome characteristics and expression pattern of NLP gene in quinoa, nine members of quinoa NLP gene were identified via bioinformatics method, and their physical and chemical properties, chromosome location, gene structure, protein conserved domain, conserved motif, systematic evolution and expression profile under different nitrogen levels were analyzed. The results showed that 9 NLP genes of quinoa were distributed on 7 chromosomes, each member contained 4-5 exons, 3-4 introns and up-stream untranslated region. NLPs promoter contained a large number of cis-acting elements responding to hormone and stress, in which a nitrogen response element GCN4 was predicted in the promoter regions of CqNLP3 and CqNLP4, respectively. The length of quinoa NLP protein was 718-952 aa, the relative molecular weight was 80.48-104.86 ku, and the isoelectric point was 5.31-6.50. Each member contained 2 conserved domains, RWP-RK and PB1, and their site on the protein were highly similar. The analysis of conserved motifs further confirmed the conservation of these two domains. Phylogenetic analysis showed that 9 NLPs could be divided into three groups, Class Ⅰ, Class Ⅱ and Class Ⅲ, and they showed closer relationship to Arabidopsis thaliana. The expression of CqNLP2, CqNLP3, CqNLP5 and CqNLP8 was depressed under nitrogen deficiency treatment, but induced under low nitrogen condition. The expression of CqNLP9 increased sharply in the late stage of low nitrogen treatment. The expression pattern of CqNLPs with qRT-PCR was consistent with that of transcriptome data. The results of this study would provide a reference basis for subsequent CqNLPs gene cloning and nitrogen stress analysis.

Key words: Chenopodium quinoa Willd., NLP transcription factor, Bioinformatics, Gene expression, Nitrogen stress

CLC Number:

S519

ZHU Manxi, ZHANG Yurong, YANG Yashu, YANG Xiaolan, WANG Chuangyun, DENG Yan, ZHAO Li, ZHANG Liguang, QIN Lixia, YANG Liyan. Identification and Expression Analysis of NLP Transcription Factor Family of Chenopodium quinoa Willd.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(4): 37-46. doi: 10.7668/hbnxb.20191913.

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