Abstract: The overexpression of Serine/Threonine protein kinase gene AtSTK can significantly enhance the salt tolerance of Arabidopsis thaliana. In order to study the regulation mechanism of AtSTK gene expression,the promoter sequences of AtSTK were amplified from genomic DNA and constructed into the reporter vector pAbAi. The recombinant plasmid pAbAi-HYT was linearized by Bstb Ⅰ and transformed into the yeast strain Y1H Gold,the linear pAbAi-HYT integrated into the genomic DNA. Then,the purified double strand cDNA of Arabidopsis and the linearized library vector pGADT7-Rec were co-transformed into the yeast cell which containing reporter vector pAbAi-HYT. The positive clones were screened on the SD/-Leu medium containing 100 ng/mL AbA by the yeast one hybrid method. Through the identification,it was found that four genes might be involved in the regulation of AtSTK gene expression. The sequencing results indicated that the Arabidopsis thaliana gene AT3G32090,which contained a conserved domain of WRKY transcription factor,was a member of the WRKY transcription factor. Therefore,the expression level of AtSTK might affect the salt tolerance of Arabidopsis plants through the MAPK signaling pathway transcription factor which coded by the gene AT3G32090.

Key words: AtSTK, Promoter, Expression regulatory proteins, Yeast one hybrid

CLC Number: 

• Q756