Analysis of qPCR and Resistance to Aphid of NTL1 Gene from Narcissus tazetta var. chinensis in Nicotiana alata

doi:10.7668/hbnxb.2017.04.013

Abstract

Abstract: The expression of the lectin gene (NTL1) from Narcissus tazzetta var. chinensis in Nicotiana alata was studied by qPCR method in order to determine the aphid-resistant function of NTL1 and cultivate N. alata with aphid-resistant. By means of susceptible to aphid,inoculation in vivo and in vitro,the resistance to aphid in different transgenic tobacco was studied. The result showed that NTL1 gene had been integrated into the tobacco,but the expression of different tobacco was different,the expression of NTL1 in tobacco by descending order was 3,4,1,2. The bioassay of aphid indicated that transgenic tobacco had a certain resistance to aphid,but the capacity of aphid-resistance in different tobacco plant was slightly different;The average density inhibition rate of aphid in inoculation in vivo and in vitro was 8.22%-76.61% and 4.62%-65.65%. The transgenic tobacco could speed up the aphid's death. The relative expression of NTL1 gene in different transgenic tobacco and the result of resistance to aphid were compared,showed that two results came with a high degree of consistency.

Key words: Narcissus tazetta var. chinensis, Lectin gene, qPCR, Nicotiana alata, Resistance to aphid

CLC Number:

S433.1

YANG Huimiao, CHEN Duanfen. Analysis of qPCR and Resistance to Aphid of NTL1 Gene from Narcissus tazetta var. chinensis in Nicotiana alata[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(4): 78-84. doi: 10.7668/hbnxb.2017.04.013.

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