Molecular Cloning of a Lectin Gene from Narcissus tazetta var. chinensis and Characteristic Prediction

doi:10.7668/hbnxb.2009.02.010

Abstract

Abstract: A new lectin gene ( NTL1)was cloned from Narcissus tazetta var. chinensis′Jinzhanyintai′.The results of sequence analysis indicated that the cDNA was 609 bp in length with an open reading frame (ORF)which was capable of encoding 172 amino acids;the protein precursor composed of a signal peptide with 25 amino-acid residue and a mature protein with 148 amino-acid residues,and its molecular weight was 18 712131 Da. The homologous analysis showed that the identity between NTL1 and Narcissus hybrid cultivar lectin,Galanthus nivalis lectin and Lycoris radiata lectin was 83 %,81 % and 77 % respectively.Characteristic prediction results indicated that the secondary structure of NTL1 includedα-helix,random coil and extended strandmainly,and its three - dimensional structure strongly resembled the snowdrop lectin,therefore the NTL1 should belong to bulb - type mannose specific binding lectin

Key words: Narcissus tazetta var. chinensis, Lectin gene (NTL1) , Sequence analysis, Characteristic prediction

CLC Number:

S682.2+1

YU Xiao-qing, CHEN Duan-fen. Molecular Cloning of a Lectin Gene from Narcissus tazetta var. chinensis and Characteristic Prediction[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(2): 47-50. doi: 10.7668/hbnxb.2009.02.010.

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