Cloning and Sequence Analysis of RcDELLA Gene in Castor

doi:10.7668/hbnxb.2018.01.003

Abstract

Abstract: DELLA family of proteins is an important repressor of plant GA signaling pathway, which can inhibit the growth and development of plants and make the plants have a dwarf phenotype. To clarify the function and function of DELLA gene in castor, conduct biological information analysis. Castor 2129 six-leaf stage shoot tip cDNA as a template,primers were designed according to the DELLA (XM_002533984.2) protein gene CDS published by NCBI, the gene was cloned and analyzed by bioinformatics. The complete reading frame of this gene was cloned,the full length of this gene was 1 701 bp, encoded 567 amino acids, molecular weight was 62 550.40 ku,isoelectric point was 5.14.The predicted secondary structure α-helix accounted for 40.4%,β-fold accounted for 8.6%,other non-random coil accounted 51.0%.Phylogenetic tree analysis showed that RcDELLA first formed a branch with Euphorbiaceae rubber tree and Jatropha curcas. The complete reading frame of RcDELLA gene was amplified by PCR, the homology comparison showed that RcDELLA had the typical DELLA domain and had high homology with the DELLA protein sequence of other plants published on NCBI. Phylogenetic tree analysis showed that the genetic relationship with Jatropha curcas was the closest and the result was consistent with species evolution.Bioinformatics analysis will lay the theoretical foundations for further study on the action and function of DELLA gene in castor dwarfing process.

Key words: Castor, DELLA, Sequence analysis, Phyletic evolution

CLC Number:

S565.03
Q78

LI Wei, LI Guorui, HUANG Fenglan, CONG Anqi, LI Xiaochen, BAI Yingjun, LI Mengjian, CHEN Yongsheng. Cloning and Sequence Analysis of RcDELLA Gene in Castor[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(1): 14-19. doi: 10.7668/hbnxb.2018.01.003.

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