Cloning and Expression Analysis of Cold Response Gene GhJAZ1 from Gossypium hirsutum L.

doi:10.7668/hbnxb.2018.01.002

Abstract

Abstract: To further study the mechanism of cotton JAZ genes in low temperature response,a cold response gene GhJAZ1 was cloned from the upland cotton Zhongmiansuo 36 by RT-PCR (GenBank accession number KJ562212).The CDS sequence of GhJAZ1 was 810 bp in length and encoded 270 amino acids.The predicted molecular weight of this protein was 29.808 ku,and the theoretical isoelectric point was 8.33.It was not a transmembrane protein.The encoded protein contained a N-terminal NT domain,a TIFY domain,and a C-terminal Jas domain.Phylogenetic tree analysis showed that GhJAZ1 was much closer to that in Theobroma cacao and Corchorus olitorius.Quantitative real-time PCR analysis showed that GhJAZ1 had significantly high expression in hypocotyls and roots.In addition,the expression of GhJAZ1 was also induced by abscisic acid(ABA) and methyl jasmonate (MeJA).The trend of expression was first raised and then decreased. Under MeJA treatment, the gene expression varied more obviously. The expression pattern of GhJAZ1 varied in different cold resistant cotton materials under cold stress. The up-regulated expression of GhJAZ1 gene in cold-resistant cotton variety-Zhongmiansuo 36 was more significant, which suggesting that GhJAZ1 gene may be involved in cold response in cotton. Subcellular localization showed that GhJAZ1 protein was localized in the nucleus.This study lay foundation for further researches on the molecular mechanism of GhJAZ1 in cold response.

Key words: Gossypium, GhJAZ1, Gene expression analysis, Cold stress, Subcellular localization

CLC Number:

Q78
S567.03

CAI Xiao, ZHEN Junbo, JIANG Zhenxing, LIU Linlin, LIU Di, ZHANG Jianhong, TIAN Haiyan, ZHANG Xiangyun, CHI Jina. Cloning and Expression Analysis of Cold Response Gene GhJAZ1 from Gossypium hirsutum L.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(1): 7-13. doi: 10.7668/hbnxb.2018.01.002.

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