Subcellular Localization and Expression Analysis of CiATAF1 Gene in Caragana intermedia

doi:10.7668/hbnxb.201751243

Abstract

Abstract: To study the expression of NAC under abiotic stress, CiATAF1 gene from Caragana intermedia was cloned and analyzed. It had an ORF of 873 bp and encoded 291 amino acids, consisting of two introns and three exons, and presenting a typical NAC domain. The genome walking method was used to obtain the CiATAF1 promoter with a length of 826 bp, which contained various response elements to light, hormone and anaerobic induction. The subcellular localization was conducted by analysis of the transient expression of Arabidopsis leaf protoplasts, showing that the CiATAF1 gene was mainly located in the nuclei. Quantitative fluorescence RT-PCR analysis showed that the expression of CiATAF1 was tissue-specific, having the highest expression level in the leaves of Caragana intermedia and relatively low level in roots and stems. Under the treatments of drought, salt, cold and ABA, the expression level of CiATAF1 increased differentially. It was speculated that the CiATAF1 gene is related to the abiotic stress regulations. Multiple sequence analysis was carried out between CiATAF1 with homologues of Arabidopsis and other leguminous plants, and their consistency of the sequences was 81.14%, indicating that the ATAF1 was relatively conservative. Bioinformatics analysis showed that CiATAF1 protein was an unstable hydrophilic protein, and its secondary structure was composed of alpha helixes, beta sheets and random coils. This study had the significance for excavating genes resistant to abiotic stress in Caragana intermedia and for analyzing the mechanism of environmental stresses in depth.

Key words: Caragana intermedia, CiATAF1, Gene expression, Subcellular localization, Abiotic stresses

CLC Number:

Q78
S793.3

WANG Yanfei, HONG Geriqiqige, WANG Guangxia, WANG Ruigang, LI Guojing. Subcellular Localization and Expression Analysis of CiATAF1 Gene in Caragana intermedia[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(3): 23-30. doi: 10.7668/hbnxb.201751243.

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