Cloning，Bioinformatics and Expression Analysis of FvARF5 Gene in Strawberry

doi:10.7668/hbnxb.201751324

Abstract

Abstract: Auxin response factor ARF is a new class of transcription factors. They play an important role during plant development as an activation or inhibition in auxin-related gene expression. In order to identify the function of FvARF5 gene during strawberry development, FvARF5 gene was cloned by RT-PCR from Fragaria vesca and analyzed by bioinformatics. Furthermore, Real-time quantitative PCR(qPCR) assay was performed to investigate the tissue-specific expression of FvARF5 gene, and its expression patterns under various phytohormone treatments. The results showed that the open reading frame of FvARF5 gene was 2 745 bp, encoding a protein of 914 amino acids. The molecular weight of FvARF5 protein was 100.65 ku, and the theoretical pI was 5.25. Multiple sequence alignment and phylogenetic tree analysis showed that FvARF5 was closely related to ARF5 from Rosa chinensis with 95% identity. FvARF5 was predicated to be the auxin response gene that was located in the nucleus. Promoter analysis revealed that the sequence of FvARF5 contained multiple hormone response elements. Real-time PCR analysis showed that FvARF5 had specific expression difference in different organs, and it had a higher expression in stem and green fruit. Also, FvARF5 had significant response to exogenous IAA and GA treatment. The results showed that the FvARF5 might play an important role in auxin and GA signal transduction during strawberry development. This research provided a theoretical basis for further study of the function and molecular regulation mechanism of FvARF5.

Key words: Strawberry, FvARF5, Auxin, Gene clone

CLC Number:

Q78
S661.1

SU Liyan. Cloning，Bioinformatics and Expression Analysis of FvARF5 Gene in Strawberry[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(3): 16-22. doi: 10.7668/hbnxb.201751324.

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