Expression and Regulation Analysis of GhTFL1b Gene in Upland Cotton

doi:10.7668/hbnxb.201751715

Abstract

Abstract: In order to study the response of cotton phosphatidylethanolamine-binding protein GhTFL1b gene to stress and physiological function, we cloned the GhTFL1b gene from upland cotton, and then analyzed its expression pattern and protein interaction. The results of promoter structure prediction using PlantCARE software revealed that GhTFL1b promoter contained MeJA responsiveness element, abscisic acid(ABA) responsiveness element, MYB binding sites involved in drought inducibility and light responsiveness elements. Meanwhile, Quantitative Real time PCR result indicated that the expression profile of GhTFL1b was induced by salicylic acid(SA) negatively, while it could be regulated by salt(NaCl) positively. Yeast two hybrid approach were under taken to elucidate the protein interaction of GhFD and GhTFL1 subgroup, and the experiments showed that GhFD could interact with GhTFL1b but not with GhTFL1a, GhTFL1b and GhTFL1c in cotton. Taken together, the result suggested that GhTFL1b might be involved in the regulation of response to abiotic stresses(SA and salt).

Key words: Upland cotton, GhTFL1b gene, Expression analysis, Regulation

CLC Number:

S562

ZHANG Xiaohong, WANG Hantao, WANG Congcong, ZHANG Fangfang, DENG Yan, WEI Hengling, HU Genhai. Expression and Regulation Analysis of GhTFL1b Gene in Upland Cotton[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(3): 9-15. doi: 10.7668/hbnxb.201751715.

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