Abstract: PP2C is an important group of protein phosphatase, which is widely involved in different adverse stress responses. In order to understand the functions of cotton PP2C genes in drought response, GaPP2C24 gene was cloned from G. arboreum cultivar Shixiya-1 by RT-PCR. Bioinformatics analysis of this gene were carried out and its expression patterns in different tissues and in drought stress response were studied by quantitative Real-time PCR. The results showed that the CDS sequence of GaPP2C24 gene was 1 251 bp in length and encoded 416 amino acids. Multiple sequence alignment showed that GaPP2C24 had a high similarity with amino acid sequences of other plants. The similarity between protein GaPP2C24 and cacao tree (EOY33930.1) and jute (OMO91132.1) was 85% and 84%, respectively. Phylogenetic tree analysis revealed that GaPP2C24 was much closer to jute that of the same genus malva, followed by cacao tree. The results of Real-time quantitative PCR showed that the expression of GaPP2C24 could be detected in cotyledons, hypocotyls, radicles, leaves, stems and roots. Its expression level was the highest in stems, followed by roots. The expression of GaPP2C24 was induced by drought stress. The expression of GaPP2C24 in roots and leaves reached the highest level after 1 h of drought treatment, with 53.9 and 6.9 times of the control, respectively. The results suggested that GaPP2C24 gene played an important role in cotton drought response.

Key words: Gossypium arboreum, GaPP2C24, Gene clone, Drought stress, Expression analysis

CLC Number: 

• Q78

• S562