Expression Analysis of PPDK2 Gene under Abiotic Stress in Foxtail Millet

doi:10.7668/hbnxb.20190885

Abstract

Abstract: Foxtail mille is a C₄ gramineous crop with drought resistance, barren-tolerance, wild adaptability and high adverse resistance. To reveal the role of the genes expression characteristics under light and stress of foxtail millet, the genes related to abiotic stress were explored in this experiment. A PPDK gene, named as SiPPDK2, was identified in foxtail millet genome by bioinformatics method. SiPPDK2 was located on chromosome 3 in foxtail millet genome, which contained 18 introns. It contained 3 transcripts and primary alternative transcript encoding 945 amino acids residues. Subcellular localization predictions indicated that SiPPDK2 was mainly localized in chloroplasts. Functional domain and multiple sequence alignment analysis revealed that SiPPDK2 protein had a more closely relationship with PPDK proteins in maize, sorghum and rice. RT-PCR analysis showed that the expression of SiPPDK2 at its seedling stage was induced by PEG, ABA, salt and low temperature treatments. Further studies indicated that SiPPDK2 was involved in drought stress and different light intensity at the jointing, heading and filling stage, in which the expression was significantly increased under the drought condition at heading and filling stages and under the low light condition at jointing and filling stage. cis-elements analysis revealed that hormonal, stress, light, and other growth-related responses cis-elements were present in the promoter region of the SiPPDK2. The results speculated that SiPPDK2 is involved in the response to abiotic stress in foxtail millet.

Key words: Foxtail millet, Pyruvate orthophos-phate dikinase(PPDK), Abiotic stress, Expression analysis

CLC Number:

S515

DU Yanwei, WANG Gaohong, LI Yanfang, ZHAO Genyou, YAN Xiaoguang, WANG Zhenhua, WANG Yuwen, YU Aili, ZHAO Jinfeng. Expression Analysis of PPDK2 Gene under Abiotic Stress in Foxtail Millet[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(3): 40-47. doi: 10.7668/hbnxb.20190885.

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