Abstract: Soybean GmVE2 gene is a NAD(P)H dehydrogenase, which is mainly present in thylakoid membranes of chloroplasts of higher plants and can participate in oxidative stress reactions and cyclic PS Ⅰ electron circulation.In order to explore its function, the full-length CDS sequence of GmVE2 gene was cloned from Dongnong 50 by RT-PCR. Use ExPASy-ProtParam and other online software to analyze the physical and chemical properties of the GmVE2 protein sequence.The prediction results showed that the coding region of the GmVE2 gene encoded a total of 167 amino acids, the molecular formula was C₉₀₄H₁₃₂₆N₂₀₈O₂₄₆S₁₀, the molecular weight was 19.364 3 ku, the total number of atoms was 2 694, the theoretical isoelectric point (pI) was 4.78, the fat index was 87.01; the residue bases of positive and negative charges were 11 and 23, respectively. The instability coefficient and GRAVY value of GmVE2 protein were 25.02 and 0.043, respectively, indicating that the protein was a stable hydrophobic protein; the protein had a transmembrane structure, a signal peptide, and a phosphorylation site.The linear plant expression vector was ligated with the target fragment and the pCambia3300-GmVE2 plant expression vector was successfully constructed. The qRT-PCR technology and high performance liquid chromatography (HPLC) were used to determine the gene expression and vitamin E content in dynamic grain and tissue, respectively. The results showed that the gene expression was negatively correlated with the vitamin E content in the growing period grains(-0.951^*), and the vitamin E content was significant higher in green tissues than in other tissues. This study conducted a preliminary study on the regulatory relationship between soybean gene GmVE2 and vitamin E content, providing a theoretical and practical basis for the selection of new soybean varieties with high soybean vitamin E content, and providing a scientific basis for later research on the function of GmVE2 gene.

Key words: Soybean, Vitamin E, GmVE2 gene, Gene cloning, Functional prediction, Expression analysis

CLC Number: 

• S565.03

• Q78