Cloning and Expression Analysis of Auxin Response Gene FvIAA17 in Strawberry

doi:10.7668/hbnxb.201751591

Abstract

Abstract: Aux/IAA protein plays an important role in regulating plant development as a transcriptional inhibitor. In order to identify the function of FvIAA17 gene in strawberry development, it was cloned by RT-PCR from strawberry. The sequence feature of FvIAA17 gene was analyzed by bioinformatics methods, and its expression patterns were analyzed by Real-time quantitative PCR(qPCR)in different tissues and under different phytohormone treatments. The results showed that FvIAA17 contained a 594 bp open reading frame and encoded 197 amino acid, with a molecular mass of 21.85 ku and an isoelectric point of 7.56. The amino acid sequence alignment showed that FvIAA17 had the typical structure domain and conservative basic sequence of Aux/IAA gene families. Promoter analysis revealed that the sequence of FvIAA17 contained the related response elements to auxin, ABA, MeJA and other stresses. Phylogenetic tree analysis indicated that FvIAA17 was most closely related to MdIAA1 from Malus domestica. FvIAA17 was an auxin response gene and was located in periplasmic space according to subcellular localization. Real-time PCR analysis showed FvIAA17 had specific expression differences in different organs, and it could be induced by exogenous IAA and ABA significantly, indicating that FvIAA17 might play an important role in auxin and abscisic acid signal transduction during strawberry development. This research provided a theoretical basis for further studying the function and molecular regulation mechanism of FvIAA17.

Key words: Strawberry, Auxin, FvIAA17, Gene clone

CLC Number:

Q78
S668.03

SU Liyan. Cloning and Expression Analysis of Auxin Response Gene FvIAA17 in Strawberry[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(4): 46-52. doi: 10.7668/hbnxb.201751591.

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