Analysis of Prickle Development Genes in Eggplant Calyx via Transcriptome Sequencing

doi:10.7668/hbnxb.201751584

Abstract

Abstract: To explore genes involved in prickle development in eggplant calyx and provide the basic knowledge for the mechanism and gene mapping of prickle development in eggplant calyx, two inbred lines, of which 1607 is the line with prickles while 1608 is not, were used to construct the F₂ population. The two parental lines and two separate pools were then used for RNA sequencing. A total of 409 363 358 clean reads were obtained, of which 311 756 616 were aligned to the eggplant reference genome, with the alignment efficiency over 75.00%. A total of 24 310 new transcripts were found and 1 540 of them were annotated. 2 438 genes were differentially expressed between the two parental lines and the two pools, of which 97 were also shared between the two pools. Based on gene annotation, three genes might be involved in the formation of prickle:SmCKX (Eggplant_newGene_1802), SmSTS (Sme2.5_09750.1_g00002)and SmFAR (Eggplant_newGene_4241). SmCKX is acytokinin oxidase/dehydrogenase, SmSTS is a stachyose synthase, and SmFAR is a fatty acyl-CoA reductase. Their homologues function in cytokinin synthesis, stachyose metabolism and wax biosynthesis pathways, individually, indicated that the development of prickle in eggplant calyx might be also affected by the aforementioned signaling.

Key words: Eggplant, Prickle in eggplant calyx, Transcriptome, Gene

CLC Number:

S641.103

QIAN Zongwei, CHEN Haili, CUI Yanling. Analysis of Prickle Development Genes in Eggplant Calyx via Transcriptome Sequencing[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(4): 53-61. doi: 10.7668/hbnxb.201751584.

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