Abstract: The vacuolar type H⁺-ATPase(VHA)is a multi-subunit endomembrane proton pump and plays a important role in plant adaptation and resistance to abiotic stresses. In order to explore the function of VHA subunit c gene(VHA-c1)in plant growth and signal transduction, the designed double strand DNA fragment was integrated into the genome of Arabidopsis thaliana by Agrobacterium mediation, and then a transgenic line c1-1 was screened to specifically silence the coding gene of VHA-c1 subunit. The results showed that the relative root length and the germination rate of seeds in c1-1 line decreased compared with the wild strain under the stress of NaCl. Especially, under the stress of 50,100 mmol/L NaCl, the relative root length of c1-1 line was suppressed sharply. Under the stress of ABA or glucose, the relative root length of c1-1 line and wild strain was suppressed. However, the relative root length of wild strain decreased more obviously than c1-1 line at 4,8 μmol/L ABA treatment. It suggested that the silenced VHA-c1 gene significantly reduced the tolerance of Arabidopsis to NaCl, and was insensitive to ABA and glucose stresses. This might be due to the different regulatory functions of H⁺-ATPase in salt stress and ABA signaling pathways.

Key words: Vacuolar type H⁺-ATPase, RNA interference technology, Abiotic stresses, Arabidopsis thaliana

CLC Number: 

• Q945.78