Response of Phosphoenolpyruvate Carboxylase(PEPC) Gene to Abiotic Stresses in Foxtail Millet

doi:10.7668/hbnxb.201751614

Abstract

Abstract: This study aims to investigate the response of foxtail millet phosphoenolpyruvate carboxylase gene under abiotic stress. The SiPEPC(Seita.1G020700)gene was identified from the foxtail millet genome, and the amino acid sequence, protein characteristics, function, signal pathway, homeopathic response components and other parameters of SiPEPC were analyzed and predicted using corresponding software. Subsequently, the dynamic expression pattern of the gene at seedling stage under the stress and its expression at jointing, heading and filling stages under different light treatments and drought stress were analyzed. The results indicated that SiPEPC was located on chromosome 1 of foxtail millet. The sequence analysis showed that SiPEPC had a length of 6 652 bp, encoding 965 amino acid residues. SiPEPC had no alternative splicing and intron. Functional domain analysis showed that SiPEPC contained the characteristic domains of PEPC gene. Multiple sequence alignment revealed that the SiPEPC protein had a very conserved sequence, which was very similar to PEPC proteins in other plants. qRT-PCR analysis showed that the expression of SiPEPC (Seita.1G020700)was up-regulated under ABA, low temperature, PEG and high salt stress treatments. Under ABA treatment, the expression level fluctuated and reached to peak at 12 h. Under low temperature treatment, the expression level continued to rise and reach to the highest at 24 h. The overall trend of expression increased when treated with PEG and NaCl, and peaked at 12 h, but decreased sharply at 24 h. Further studies showed that SiPEPC gene participated in the response to drought stress under normal light intensity at jointing and heading stages. These results suggested that SiPEPC (Seita.1G020700)gene participated in the response to abiotic stress and might play a key role in signaling pathway of drought or other stresses.

Key words: Foxtail millet, Phosphoenolpyruvate carboxylase gene, Adversity stress, Response

CLC Number:

Q78
S515

ZHAO Jinfeng, WANG Gaohong, DU Yanwei, LI Yanfang, WANG Zhenhua, ZHAO Genyou, YU Aili. Response of Phosphoenolpyruvate Carboxylase(PEPC) Gene to Abiotic Stresses in Foxtail Millet[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(4): 67-74. doi: 10.7668/hbnxb.201751614.

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