The Composition and Difference Analysis of Endophytic Bacterial Community in Different Resistant/Susceptible Foxtail Millet Varieties

doi:10.7668/hbnxb.20193151

Abstract

Abstract:

In order to clarify the characteristics of the endophytic bacterial community of different varieties of foxtail millet,reveal the key species related to foxtail millet downy mildew resistance.DNA extraction was performed on the lowest node stem and the highest node stem tissues of 3 kinds of foxtail millet downy mildew-resistant and 3 kinds of foxtail millet downy mildew-susceptible foxtail millet plants.We used Illumina Miseq to sequence the bacterial 16S rDNA of the samples and analyzed the susceptible community composition of endophytic bacteria.The results showed that at each classification level,the endophytic bacterial species from the lowest node stem to the highest node stem showed two different trends in disease-resistant and susceptible foxtail millet samples,which were basically stable and significantly decreased.And the species composition of the endophytic bacterial community in disease-resistant foxtail millet was more abundant than that of susceptible foxtail millet.19 important endophytic bacteria phyla were obtained:(from the comparison of the endophytic bacteria ubiquitous in the lowest node stem of disease-resistant and susceptible foxtail millet samples were obtained) 2 species of endophytic bacteria phyla specific to disease-resistant foxtail millet;(from the comparison of all endophytic bacteria of disease-resistant and susceptible foxtail millet samples 16 species of endophytic bacteria phyla unique to disease-resistant foxtail millet and 2 dominant groups with relatively large abundance of disease-resistant foxtail millet were obtained).It can be seen that the endophytic bacterial communities in different foxtail millet varieties and different parts are different,and the endophytic bacterial species of disease resistance are more abundant than that of susceptible foxtail millet.Among them,the endophytic bacteria phyla unique to disease-resistant foxtail millet materials and the dominant endophytic bacteria phyla with relatively large abundance may have an effect on the prevention and treatment of foxtail millet downy mildew,which is worthy of further study.

Key words: Foxtail millet, Downy mildew, Endophytic bacteria, Miseq high-throughput sequencing

ZOU Xiaoyue, LIU Jia, DONG Zhiping, MA Jifang, SHI Aili, BAI Hui, LI Zhiyong. The Composition and Difference Analysis of Endophytic Bacterial Community in Different Resistant/Susceptible Foxtail Millet Varieties[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(S1): 300-308. doi: 10.7668/hbnxb.20193151.

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Figures/Tables 7

Tab.1 Amplification primer sequence

巢式PCR Nested PCR	引物 Primer	序列(5'-3') Sequence(5'-3')	扩增区域 Target region
第1轮	799F	AACMGGATTAGATACCCKG	16S rDNA
First round	1392R	ACGGGCGGTGTGTRC	799F-1392R
第2轮	799F	AACMGGATTAGATACCCKG	16S rDNA
Second round	1193R	ACGTCATCCCCACCTTCC	799F-1193R

Fig.1 Coverage index of foxtail millet samples R.Disease-resistant;S.Disease-susceptible;LS.The lowest node stem;HS.The highest node stem;1,2,3.The material number.

Fig.2 Shannon dilution curve of foxtail millet samples

Tab.2 Species of endophytic bacteria in resistant and susceptible samples of foxtail millet at different classification levels

分类水平 Classification level	门 Phylum	纲 Class	目 Order	科 Family	属 Genus	种 Species
R_LS	23	40	97	224	423	647
R_HS	27	47	106	218	421	606
S_LS	19	35	90	202	345	513
S_HS	11	17	48	94	163	238

Fig.3 Comparison of endophytic bacteria phylum in the lowest node stem of foxtail millet samples A.Venn diagram of distribution of endophytic bacteria phyla in the lowest node stems of disease-resistant foxtail millet samples;B.Venn diagram of the distribution of endophytic bacterial phyla in the lowest node stems of disease-susceptible foxtail millet samples;C.Venn diagram of distribution of endophytic bacterial phyla in the lowest node stems of disease-resistant and susceptible foxtail millet samples;D.Histogram of distribution of dominant endophytic bacteria phyla in the lowest node stems of disease-resistant and susceptible foxtail millet samples.R1.Shanxihonggu;R2.Changnong 46;R3.Zheng 17-3415;S1.Zheng 17-2123;S2.K148-1;S3.Baogu 25;R.3 resistant samples of foxtail millet;S.3 susceptible samples of foxtail millet;LS.The lowest node stem.The same as Fig.4-5.

Fig.4 Comparison of endophytic bacteria phylum in the highest node stem of foxtail millet samples A.Venn diagram of distribution of endophytic bacteria phyla in the highest node stems of disease-resistant foxtail millet samples;B.Venn diagram of the distribution of endophytic bacterial phyla in the highest node stems of disease-susceptible foxtail millet samples;C.Venn diagram of distribution of endophytic bacterial phyla in the highest node stems of disease-resistant and susceptible foxtail millet samples;D.Histogram of distribution of dominant endophytic bacteria phyla in the highest node stems of disease-resistant and susceptible foxtail millet samples.HS.The highest node stem.The same as Fig.5.

Fig.5 Comparison of endophytic bacteria phylum in different parts of foxtail millet samples A.Venn diagram of the distribution of endophytic bacterial phyla in the lowest node stems and highest node stems of disease-resistant foxtail millet samples;B.Venn diagram of the distribution of endophytic bacterial phyla in the lowest node stems and highest node stems of disease-susceptible foxtail millet samples;C.Histogram of the distribution of dominant endophytic bacteria phyla in the lowest node stems and highest node stems of disease-resistant and susceptible foxtail millet samples.

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