Identification of the Acid Phosphatase-encoding Family Genes in Foxtail Millet and SiACP1 Haplotype Analysis with Low Phosphorus Tolerance

doi:10.7668/hbnxb.20191202

Abstract

Abstract: Intracellular acid phosphatase (ACP) is an enzyme which hydrolyzes phospholipids in vacuoles to inorganic phosphorus. The ACP enzyme is also ubiquitous in plant tissues and plays an important role in regulating plant phosphorus nutrition. It will be one of the main genetic and breeding approaches to solve the deficiency of available phosphorus in soil by developing new varieties assisted superior alleles and haplotypes in SiACP genes. First identified 13 ACP family genes form foxtail millet and other three gramineous crops by homologous alignment. After systematic bioinformatics analysis,it was found that the number and distribution of ACP family genes in gramineous crops had certain regularity,and they all had highly conserved phosphatase domains and motifs. In addition,through the analysis of family evolutionary tree,it was found that the ACP genes of monocotyledons and dicotyledons had obvious branches in the evolutionary process. Finally,expression pattern analysis of transcriptome data showed that SiACP1 genes in foxtail millet apparently exhibited a tissue specific expression,while the higher expression of SiACP2 and SiACP3 genes was found in different tissues through the growth and development stage. Meanwhile,through candidate gene association analysis and haplotype analysis,SNPs located in SiACP1 promoter was significantly associated with the traits with low phosphorus tolerance. Interestingly,a haplotype ACPHap2 which was favorable to low phosphorus tolerance of foxtail millet was preliminarily identified. Research results will provide some genetic information reference for the discovery of excellent germplasm with low phosphorus tolerance and molecular breeding of crops in further.

Key words: Foxtail millet, ACP family gene, Low phosphorus tolerance, Haplotype

CLC Number:

S515.03
Q78

ZHAO Xiongwei, WU Nianlong, QIAO Jiahui, LI Xukai, HAN Yuanhuai, XING Guofang. Identification of the Acid Phosphatase-encoding Family Genes in Foxtail Millet and SiACP1 Haplotype Analysis with Low Phosphorus Tolerance[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(4): 35-45. doi: 10.7668/hbnxb.20191202.

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