Molecular Marker Detection and Analysis of Allelic Variation Combinations of Grain Weight Genes Based on Introduced Wheat Germplasm Resources

doi:10.7668/hbnxb.20192106

Abstract

Abstract: In order to effectively utilize the introduced wheat germplasm resources and explore the breeding application value of some wheat grain weight genes, the 1000-kernel weight(TKW) of 48 introduced wheat germplasms were identified, and the allelic variation types and the distribution of high TKW alleles were detected by using the functional molecular markers of four grain weight genes(TaGW2-6A、TaCwi-A1、TaGS2-D1 and TaSus2-2B). The results showed that the average TKW of the introduced germplasm materials was 30.15 g, the variation range was 20.13-43.19 g, and the germplasm materials above the average accounted for 45.8%. There were 8 haplotypes in the 4 grain weight-related genes in the introduced wheat germplasms. The average TKW of high TKW haplotypes TaCwi-A1a of TaCwi-A1 gene and Hap-6A-A of TaGW2-6A gene were significantly higher than the corresponding low ones(TaCwi-A1b and Hap-6A-G). The average TKW of the low TKW haplotypes TaSus2-2Bb of TaSus2-2B gene and TaGS2-D1b of TaGS2-D1 gene were significantly higher than the corresponding high TKW haplotypes TaSus2-2Ba and TaGS2-D1a. Generally germplasm materials containing the same haplotype combination of each grain weight gene accounted for a relatively higher proportion of the tested materials, and their average TKW was relatively higher. Studies indicate that the grain weight-related traits of these wheat germplasms have good genetic improvement potential, and it is necessary to analyze the comprehensive genetic effects of related grain weight genes in wheat breeding.

Key words: Wheat, Introduced germplasm, Grain weight gene, Molecular marker, Haplotype

CLC Number:

S512.1

WU Xiaojun, LI Fengyuan, SHEN Yunxia, CHEN Xiangdong, HU Xigui, JIANG Hao, ZHANG Xuening, LI Xiaoli, HU Tiezhu, RU Zhengang. Molecular Marker Detection and Analysis of Allelic Variation Combinations of Grain Weight Genes Based on Introduced Wheat Germplasm Resources[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(5): 127-134. doi: 10.7668/hbnxb.20192106.

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