Mapping of Drought Resistance QTLs at Germination Stage of Foxtail Millet

doi:10.7668/hbnxb.20191175

Abstract

Abstract: In order to explore the genetic basis of drought resistance and guide drought resistance breeding, mapping of QTL associated with drought resistance at germination stage of foxtail millet was done. An F₂ segregating population was constructed using 100 individuals derived from a cross between Shanxi 2010 and K359×M4-1. A genetic linkage map was constructed based on 2b-RAD sequencing, which was then combined with the phenotypic trait of drought resistance for QTL mapping. The results showed that drought resistance at germination stage of foxtail millet was a complex quantitative trait and controlled by multiple genes. A genetic map containing 583 SNP makers was constructed by 2b-RAD of parents and F₂ population. The map covered 9 chromosomes of foxtail millet, with an average number of 64.8 and an average genetic distance of 0.97 cM between markers. A total of 3 QTLs were identified:qSIDR-5a, qSIDR-6a and qSIDR-6b, located in foxtail millet chromosome 5 and 6, respectively, which explained 12.4%-14.3% of phenotypic variation. Among them, phenotypic contribution rate of qSIDR-5a was the highest, which explained 14.3% of phenotypic variation. These QTLs were not included in the same chromosome interval as the identified drought resistance related QTLs of foxtail millet, and thus were described as new candidate gene loci associated with germination stage drought resistance.These QTLs can be used for fine mapping and gene cloning, as well as molecular regulation mechanism of drought resistance of foxtail millet.

Key words: Foxtail millet, Germination stage, Drought resistance, QTL

CLC Number:

S515.01

DAI Xiaodong, ZHU Cancan, WANG Chunyi, QIN Na, SONG Yinghui, DAI Shutao, LI Junxia. Mapping of Drought Resistance QTLs at Germination Stage of Foxtail Millet[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(6): 36-41. doi: 10.7668/hbnxb.20191175.

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