Identification of QTL Involved in Anaerobic Germination Tolerance by RIL Population in Rice

doi:10.7668/hbnxb.20191206

Abstract

Abstract: Identifying rice resources with strong germination ability under anaerobic condition, and then mapping QTL related to anaerobic germination ability could provide materials and QTLs for improving the anaerobic germination ability of direct-seeded rice. The anaerobic germination ability of the indica local variety Zhaxima from Yunnan Province, the japonica variety Nanjing 46 with excellent taste from Jiangsu Province and the Zhaxima/Nanjing 46 RIL populations containing 135 lines were evaluated in centrifuge tubes (10 cm), which were filled with distilled water. The seeds were germinated at 28℃ for 7 days in a dark germination chamber, and then the length of the coleoptile of the seeds was measured and used as the evaluation standard for the anaerobic germination ability. The results showed that after 7 days of flooding, the lengths of the coleoptiles of Zhaxima and Nanjing 46 were 2.92,6.68 cm, respectively, and there was extremely significant statistical difference between the two varieties at the P=0.01 level. Furthermore, the QTL related to anaerobic germination was mapped by RIL population consisting of 135 lines derived from a cross between Zhaxima and the Nanjing 46, which revealed that one QTL(qAG-12) correlated with anaerobic germination located on chromosome 12 between SSR marker RM1300 and RM1227. The qAG-12 with a LOD score of 3.04 explained about 11.24% of the phenotypic variation. Bioinformatics analysis showed that the qAG-12 was located in the range of 1.34 Mb between 26.00,27.34 Mb on chromosome 12. There were more than 200 predicted ORFs in this candidate region of qAG-12, and no reported QTL involved in anaerobic germination at the germination stage in the region. The above results indicated:the anaerobic germination ability exhibited extremely significant difference between Zhaxima and the Nanjing 46 at the level of P=0.01; The qAG-12 was a novel QTL correlated with anaerobic germination.

Key words: Direct-seeded rice, Recombination inbred lines, Anaerobic germination, QTL mapping

CLC Number:

S511.03

ZHANG Suobing, ZHANG Yunhui, CHEN Haiyuan, LIN Jing, WANG Yingjie, ZHU Xiaomei, SONG Chunfeng, FANG Xianwen. Identification of QTL Involved in Anaerobic Germination Tolerance by RIL Population in Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(6): 31-35. doi: 10.7668/hbnxb.20191206.

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