QTL Identification of Yield-related Traits Using Four-way Recombinant Inbred Population in Soybean

doi:10.7668/hbnxb.20190471

Abstract

Abstract: The purpose of this study was to explore the correlation between soybean yield and yield-related such as plant height, main stem node number, pod number per plant, seed number per plant and 100-seed weight and to localize QTLs for controlling these traits so as to improve soybean yield.This experiment, the four-way recombinant inbred line population(FW-RIL)with 160 lines derived from four soybean parents (Kenfeng 14×Kenfeng 15)×(Heinong 48×Kenfeng 19) with significant difference in yield-related characters was used as materials and planed in Harbin (2013-2015) and Keshan (2013,2015). Obtaining phenotypic data of plant height, main stem node number,pod number per plant, seed number per plant, 100-seed weight combined with constructed genetic map containing 275 SSR markers to map QTL for yield-related traits. Experimental results showed that 28 yield-related QTLs were detected repeatedly in multi-environments and 10 of them were plant height QTLs, phenotypic variation explained was between 3.20% and 11.72%. Three main stem node number QTLs, phenotypic variation explained was 6.55%,5.70%,3.77% respectively. Nine pod number per plant QTLs, phenotypic variation explained was between 2.60% and 11.25%. Four 100-seed weight QTLs, phenotypic variation explained was between 3.83% and 9.35%.Two seed number per plant QTLs, phenotypic variation explained was 8.58%,7.52% respectively.28 repeated detected yield traits QTLs were located,16 new yield-related QTLs were detected and 12 of them were consistent with the QTLs for yield-related traits reported at home and abroad, indicating a high accuracy of QTLs detection. Using molecular marker genetic map to locate QTLs for controlling yield-related traits provides a powerful means for using molecular marker to improve soybean yield potential.

Key words: Soybean, Yield, QTL mapping, Four-way recombinant inbred population

CLC Number:

S565.03

WANG Yanshu, TIAN Yu, ZHANG Jianan, XU Shichao, DONG Quanzhong, LI Wenbin, LI Wenxia, NING Hailong. QTL Identification of Yield-related Traits Using Four-way Recombinant Inbred Population in Soybean[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(3): 79-86. doi: 10.7668/hbnxb.20190471.

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