DNA Fingerprinting and Genetic Diversity Analysis of National Maize Regional Trials in 2014-2019

doi:10.7668/hbnxb.20191055

Abstract

Abstract: With the reform of maize variety approval system and the expansion of trial channels in China, nearly 8 000 maize combinations are tested every year. In order to understand the breeding level, development trend and existing problems in recent years, 2 949 national regional trials combinations in 2014-2019 were analyzed for genetic diversity and DNA fingerprinting. With an average of 14.55 alleles per locus were detected in 40 SSR loci. The average gene diversity and PIC were 0.71 and 0.67, respectively. In 2014-2019, the genetic diversity of the trial combinations was basically the same, the lowest in the pop corn, and the highest in the fresh corn, and the increasing trend from north to south in the ecological planting area. The results of group heterotic grouping and principal coordinates analysis showed that the groups of pop corn, tropical and subtropical, fresh corn, early maturity and Southwest group belonged to different subclasses respectively. The genetic background of other groups mixed with each other, the genetic relationship between Northeast and Northwest was the closest. The results of DNA fingerprint authenticity test showed that the number of samples with less than 5 different loci compared between the combinations and database decreased year by year. In the past ten years, the genetic diversity of the combinations in the maize regional trials had been increasing, SSR technology played an important role in the regional trails, with the development of new breeding techniques, it is recommended to accelerate the research and application of new technologies such as SNP in regional trials.

Key words: Maize, National regional trial, SSR, Genetic diversity, DNA fingerprint

CLC Number:

S513.03

YI Hongmei, REN Jie, WANG Lu, WANG Rui, GE Jianrong, WANG Fengge, ZHAO Jiuran, XU Mingliang. DNA Fingerprinting and Genetic Diversity Analysis of National Maize Regional Trials in 2014-2019[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(3): 87-93. doi: 10.7668/hbnxb.20191055.

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