QTL Mapping of Low Temperature Sensitivity Affecting the Fertility Reversibility in Thermo-sensitive Genic Male Sterile Rice

doi:10.7668/hbnxb.20190431

Abstract

Abstract: The photoperiod and temperature sensitivity of fertility reversibility has an important effect on the seed propagation and hybrid seed production of photo-and thermo-sensitive genic male sterile (PTGMS) rice. The aim of this study was to map QTLs of low temperature sensitivity affecting the fertility reversibility in thermo-sensitive genic male sterile (TGMS) rice. N38S and N727S were two TGMS rice which were obvious difference in seed setting rate of cold-water irrigation propagation. Using the F₂ population of N38S×N727S treated with low temperature in the artificial climate chamber and SSR marker technology, combined with Mapmaker 3.0 and WinQTLCart 2.5 software analysis, the QTLs of low temperature sensitivity were detected. The results showed that three QTLs of low temperature sensitivity were detected. The QTL1 was mapped to the interval between PSM12 and RM583 on chromosome 1. The QTL1 and QTL2 were mapped to the interval between PSM194 and RM273 and between RM273 and PSM103 on chromosome 4, respectively.Their additive effect was all negative value. To fertility reversibility, the contribution rate of the three QTLs was 7%, 16% and 3% in turn. Location information based on SSR markers suggested that the three QTLs were not the same as other reported genes related to fertility in PTGMS rice. In conclusion, QTL1,QTL2 and QTL3 may be new gene loci. The allele from N727S plays a role in increasing the yield of seed propagation. When the three QTLs are used as molecular marker assisted selection, the molecular marker type of N727S should be selected. So the three QTLs of low temperature sensitivity mapped in this study might be of great significance for molecular marker assisted selection of TGMS rice with a stable sterility but easy to reverse to fertility in seed propagation.

Key words: Thermo-sensitive genic male sterile rice, Fertility reversibility, Low temperature sensitivity, QTL mapping

CLC Number:

S511
S334.5

LIANG Tiejun, CHEN Xionghui, CUI Yumei, LUO Fangxiong, ZHANG Zemin, PENG Haifeng. QTL Mapping of Low Temperature Sensitivity Affecting the Fertility Reversibility in Thermo-sensitive Genic Male Sterile Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(2): 29-34. doi: 10.7668/hbnxb.20190431.

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