The Antifungal and Plant Growth-Promoting Effect and Components Analysis of the Volatile Organic Compounds from an Endophytic Bacterium S258

doi:10.7668/hbnxb.20192376

Abstract

Abstract: In order to further utilization of the endophytic antagonistic bacterium S258 isolated from cotton root for biological control, especially to use the volatile organic compounds(VOCs) produced by the strain to control plant disease, Verticillium dahliae and Fusarium oxysporum f. sp. niveum were used as the target pathogenic fungi, and Arabidopsis thaliana was used as the indicator plant, the antifungal activity of the VOCs from the strain against pathogenic fungi and the growth promoting activity against plants were determined by the dual culture on two-section of a Petri dish. The results showed that the VOCs from the endophytic bacterium S258 could inhibit the mycelial growth of pathogenic fungi and at the same time promoted the plant growth. Compared with the negative control E. coli DH5α, the growth inhibition rate of the VOCs against Verticillium dahliae and Fusarium oxysporum f. sp. niveum were 80.9% and 10.1%, respectively. Compared with the blank control without bacteria, the fresh weight per plant of Arabidopsis thaliana treated by the VOCs was 5.3 times that of the blank control. The VOCs from the endophytic bacterium S258 were collected by headspace solid-phase microextraction. Then the compositions of VOCs were analyzed by GC-MS. The VOCs were identified by NIST and WILIY mass spectrometry databases as 30 monomer compounds, belonging to alkanes, alkenes, alcohols, ketones, ethers, aldehydes, esters and heterocycles. According to the peak area of total ion current graph, dimethyl disulfide and indole were most abundant among these compounds. In conclusion, the endophytic bacteria S258 can produce volatile organic compounds with antifungal and growth-promoting activity, which is an important microbial resource for the development of biocontrol products, and its volatile organic compounds also have high research and application value.

Key words: Endophytic bacteria, Volatile organic compounds, Antifungal activity, Growth-promoting activity

CLC Number:

S432.1

LUO Xiaoyu, SHAN Chenyang, JIA Zhaozhao, ZHANG Xin, LIN Ling. The Antifungal and Plant Growth-Promoting Effect and Components Analysis of the Volatile Organic Compounds from an Endophytic Bacterium S258[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(5): 198-203. doi: 10.7668/hbnxb.20192376.

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