ACTA AGRICULTURAE BOREALI-SINICA ›› 2013, Vol. 28 ›› Issue (4): 208-213. doi: 10.3969/j.issn.1000-7091.2013.04.038

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Inhibiting Effects of Endophytic Bacterial Strain JM- 3 from Agavesisalana Perrine against Valsa ceratosperma

ZHANG Shu-ying1,2, QU Tian-li3, SUN Yang4, DONG Xiang-li2, WANG Cai-xia2, LI Bao-hua2, XIAO Chun1   

  1. 1. College of Plant Protection, Yunnan Agriculture University, Kunming 650201, China;
    2. College of Agriculture and Plant Protection, Qingdao Agriculture University, Qingdao 266109, China;
    3. College of Chemistry and Pharmaceutical Sciences, Qingdao Agriculture University, Qingdao 266109, China;
    4. College of Life Sciences, Qingdao Agriculture University, Qingdao 266109, China
  • Received:2013-05-12 Published:2013-08-28

Abstract: Six isolates of endophytic bacterial strains were isolated from healthy leaves of Agavesisalana Per- rine,in which the strain JM- 3 inhibited canker lesion expansion of Valsa ceratosperma on excised twigs significantly. The cell- free fermentation liquid of JM- 3 inhibited mycelium growth and conidial germination of Valsa ceratosperma. The isolates produced anti- fungi substances,which caused malformation of mycelia and germtubes.The malformed mycelium burst and protoplasm flow- out finally.JM- 3 also showed clear inhibiting effect against mycelial growth of pathogen from several fruit trees and crop.JM- 3 was identified as Bacillus subtilis based on 16S rDNA sequencing and RiboPrinter results.

Key words: Endophytic bacteria, Bacillus subtilis, Valsa ceratosperma

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Cite this article

ZHANG Shu-ying, QU Tian-li, SUN Yang, DONG Xiang-li, WANG Cai-xia, LI Bao-hua, XIAO Chun. Inhibiting Effects of Endophytic Bacterial Strain JM- 3 from Agavesisalana Perrine against Valsa ceratosperma[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(4): 208-213. doi: 10.3969/j.issn.1000-7091.2013.04.038.

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