Screening of Wheat Endophytic Bacteria W-1 and the Inhibition to Rhizoctonia cerealis

doi:10.7668/hbnxb.2015.S1.050

Abstract

Abstract: Wheat sharpeye sport caused by Rhizoctonia cerealis is very important and difficult control disease in wheat production.Exploring new safely,high efficiency control method is an important test in this disease control.Screening and applying the endophytic bacteria is an important focus in biological control of plant disease.In order to acquire endophytic bacteria against R.cerealis and enrich the methods of control wheat sharp eyespot,the endophytic bacteria strains from wheat were isolated by using spread plate method.The antagonistic strains were screened by inhibition zone method and tablet confrontation culture method.The results showed that 4 of 8 plants endophytic bacteria strains isolated from the wheat displayed antagonistic activity to Rhizoctonia cerealis,and W-1 was the best one.The mycelia of Rhizoctonia cerealis were treated with fermentation liquid of W-1 and showed deformity,ablation and fracture.However,the wheat could grow normally treated with fermentation liquid of W-1.Colonization test showed that the isolate W-1 could colonize in wheat stably.

Key words: Wheat sharp eyespot, Endophytic bacteria, Antagonism, Screening

CLC Number:

S435.12

ZHANG Yu-mei, LI Han-bing, BI Ming-zhao, WANG Zhi, ZHANG Na, YANG Wen-xiang, LIU Da-qun. Screening of Wheat Endophytic Bacteria W-1 and the Inhibition to Rhizoctonia cerealis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(S1): 278-282. doi: 10.7668/hbnxb.2015.S1.050.

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http://www.hbnxb.net/EN/Y2015/V30/IS1/278

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