Abstract: The extension of bio-control bacteria resources is significant to the development of bio-control of grape mycosis. To efficiently acquire bio-control bacteria again grape mycosis,the key components of the cell wall of fungal pathogen,chitin and β-1,3-gluncan,were successively used as the only carbon source. Through the screening method with substrate stress,target strains with both chitinase activity and β-1,3-glucanase activity were selectively enriched. Results suggested that this screening method effectively enriched the potential target strains. All of the 10 picked strains demonstrated chitinase activity and β-1,3-glucanase activity. Among them,the chitinase activity per biomass and β-1,3-glucanase activity per biomass of strain 1# reached 2.52,0.76 U/mL respectively,and those of strain 6# reached 1.45,3.99 U/mL respectively. Enzyme study proved that the enzymes of both strains were inducible. Under simulated natural conditions,the enzyme activities of the two strains reached the highest at 24 or 36 h. Through 16S rDNA sequencing,both of the two strains were identified as Pseudomonas sp.,which is a kind of endophytic bacteria with great potential for bio-control.

Key words: Grape mycosis, Bio-control bacteria, Screening stress, Chitinase, β-1,3-glucanase

CLC Number: 

• S432.4

• Q939.92