Abstract: Chitin and β-1,3-glucan is the main constituent of most fungal cell walls,which can be degraded by chitinase(Chi) and β-1,3-glucanase(Glu).Chitinase gene(Chi) and β-1,3-glucan gene(Glu) has a synergistic effect,chitinase and β-1,3-a variety of injuries can be induced in chitin quality enzymes and β-1,3-glucanase exists in most plant cells.In normal circumstances,chitinase and β-1,3-glucanas of the plant body only has low levels of the type of constitutive expression,but in the pathogen infect,induction and a variety of injuries can be induced in chitinase and β-1,3-glucanase.Also,fungal disease is one of main diseases who do harm to plants,these diseases mainly extend to other area through transportation by phloem.If we can use vascular specifically expressed promoter adjustment purpose gene(antifungal disease gene) in the phloem efficient specific to express,it can be more effective to play the role of target gene.A vascular-specific promoter BSP cloned chitinase gene(Chi) and β-1,3-glucanase(Glu) gene,and built a BSP driver Chi and Glu gene the bivalent express carrier pBIBCG.Restructuring the plasmid pBIBCG after restriction enzyme analysis and identification,the results indicate that the vascular-specific start sub-BSP drive containing the bivalent anti-fungal gene plant expression vectors have been constructed successfully.Laid the foundation for genetic engineering breeding for plant anti-fungal,has a very important role to improve the level of overall disease resistance of plants.

Key words: Dual price vector, Vascular-specific promoter, Chitinase(Chi) β-1 3-3-glucanase(Glu)

CLC Number: 

• S188