Cloning of VP1 Gene of Chicken Infectious Anemia Virus and Its Expression in E. coli

doi:10.3321/j.issn:1000-7091.2006.05.013

Abstract

Abstract: We designed a pair of oligonucleotide primers based on the genomic sequence of CAV Cux_1 strain,and amplified VP1 gene by PCR.The amplification fragment was cloned into pGM_T easy vector.The result of nucleotide sequence analysis demonstrated that the cloned genomic DNA was identical with that deduced from Cux_1 strain of CAV.Then the cloned genomic DNA was subcloned into prokaryotic expression vector PET_32a(+),and the recombinant protein was expressed in E.coli cell.SDS_PAGE and Western Blot analysis showed that a protein with a molecular weight of 70.4 kDa was successfully expressed and CAV_infected chicken serum reacted with the recombinant protein.

Key words: Chicken infectious anemia virus, VP1, Cloning, Expression, E.coli

CLC Number:

S188

HUANG Dong-yan, YANG Bing, QI Xin, CHEN Xiao-ling, XU Fu-zhou. Cloning of VP1 Gene of Chicken Infectious Anemia Virus and Its Expression in E. coli[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(5): 50-53. doi: 10.3321/j.issn:1000-7091.2006.05.013.

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