ACTA AGRICULTURAE BOREALI-SINICA ›› 2015, Vol. 30 ›› Issue (3): 54-57. doi: 10.7668/hbnxb.2015.03.010

Special Issue: Cotton Biotechnology

Previous Articles     Next Articles

Genetic Analysis of Transgenic Glyphosate-resistance Cotton with EPSPS Gene

YAN Shu-feng1,2,3, ZHU Shui-jin2, LIU Hai-fang4, LU Cai-xia1,3, TIE Shuang-gui1,3   

  1. 1. Cereal Crops Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China;
    2. Agronomy Department, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310085, China;
    3. Henan Provincial Key Lab of Maize Biology, Zhengzhou 450002, China;
    4. Jiyuan Agriculture Bureau, Jiyuan 454650, China
  • Received:2015-02-06 Published:2015-06-28

Abstract: The purpose of this study was to analysis the genetic characters of transgenic glyphosate-resistance cotton with EPSPS gene,using the glyphosate-resistant cotton transformation events G6-1-G6-26 as the materials,and their non-transgenic genetic background cultivar CCRI-49 as control.Field resistance test in T1 plants showed that the 20 of 26 transformation events were consistent with the ratio of 3: 1 and the other transformation events were inconsistent with single-gene Mendelian inheritance according to χ2 test.Field resistance test in T2 plants showed 152 homozygous resistance lines were obtained derived from 25 transformation events,respectively.Fifty-seven lines derived from 15 transformation events were consistent with the ratio 3: 1 through the analysis of heterozygous resistance lines.Furthermore,every transformation event had lines which were inconsistent with the ratio of 3: 1,and 10 transformation events did not have lines which were consistent with the ratio of 3: 1.These suggested the integration and genetic mode of exogenous gene transformed by the pollen tube pathway method were complex.

Key words: Cotton, Glyphosate, EPSPS gene, Genetic analysis

CLC Number: 

Cite this article

YAN Shu-feng, ZHU Shui-jin, LIU Hai-fang, LU Cai-xia, TIE Shuang-gui. Genetic Analysis of Transgenic Glyphosate-resistance Cotton with EPSPS Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(3): 54-57. doi: 10.7668/hbnxb.2015.03.010.

share this article