Analysis of Oat Seed Protein during Seed Germination under Salt Stress

doi:10.7668/hbnxb.2015.03.009

Abstract

Abstract: To analysis the differences of protein expression and its related physiological function of oat seed germination under salt stress,the test compared the ability of oat seed germination and seedling growth in different concentrations of NaCl solution,and analyzed protein expression of oat seed imhibition in 0 and 1% NaCl solution by two-dimensional gel combined with matrix assisted laser analytical time of flight mass spectrometry proteomics methods.The results showed:the ability of oat seed germination and seedling growth reduced with the salt stress was heavy.Proteomic analysis found:eleven protein spots were altered under 1% NaCl solution,and ten of them protein expression quantity increased.At last,three reliable protein were got by mass spectrum identification.There are oat protein,oat protein N9 and key protein disulfide isomerase,respectively.The differences of these protein expression may be linked with the activity mechanism of oat seed germination under salt stress.

Key words: Avena sativa L., Two-dimensional gel electrophoresis, Protein, Salt stress

CLC Number:

S512.03

WANG Xia-xia, LI Yan, TANG Jie-wei, OUYANG Can-bin, ZHU De-jian, XU Zhi-ming. Analysis of Oat Seed Protein during Seed Germination under Salt Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(3): 48-53. doi: 10.7668/hbnxb.2015.03.009.

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http://www.hbnxb.net/EN/Y2015/V30/I3/48

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