Cloning,Protein Function and Tissue Expression Analysis of Yak CTGF Gene

doi:10.7668/hbnxb.20191763

Abstract

Abstract: In order to obtain the CDS region sequence of the yak CTGF gene and the structure and function of the encoded protein, and to study the mRNA expression level of this gene in tissues such as kidney, heart, lung, liver and gluteal muscle. The experiment took Leiwuqi yak as the research object. RT-PCR was used to obtain the CDS sequence of CTGF gene of Leiwuqi yak. Fluorescence quantitative PCR(qPCR)method was used to detect the mRNA expression level of this gene in five tissues. The results showed that the yak CTGF gene contained two CpG islands, the open reading frame length was 1 050 bp(accession number:MT968972), which could encode 349 amino acids, and the CTGF encoded protein had a signal peptide, which was a water-soluble unstable surface protein. There were 21 phosphorylation sites and 7 glycosylation sites, which were more distributed in the endoplasmic reticulum and microsomes(peroxisomes), with 3 complete conserved functional domains. Fluorescence quantitative results showed that CTGF gene was expressed in kidney, heart, lung, liver and gluteal muscle tissues of Leiwuqi yak, and the expression level was highest in liver. The aim was to further understand the role of CTGF gene in regulating yak muscle growth and provide reference data.

Key words: Yak, CTGF gene, Cloning, Protein function analysis, Tissue expression analysis

CLC Number:

S823.85

CHEN Mei, CHAI Zhixin, WU Zhijuan, WANG Jikun, ZHONG Jincheng, XIN Jinwei. Cloning,Protein Function and Tissue Expression Analysis of Yak CTGF Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(S1): 361-367. doi: 10.7668/hbnxb.20191763.

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