Correlation Analysis of IGFBP-3 Gene Nucleotide Variation and Ovine Slaughter Traits

doi:10.7668/hbnxb.20191949

Abstract

Abstract: Insulin-like Growth Factor Binding Proteins (IGFBPs) are closely related to the growth and development of animals.In order to explore the possibility of IGFBP-3 gene as a candidate gene for sheep slaughter traits, this study used Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP) combined with sequencing to detect variation characteristics of IGFBP-3 gene and to explore the correlation between nucleotide variation and slaughter traits in 592 Gansu alpine merino sheep.The results showed that one single nucleotide variant site (g.87 A>C) was detected in the fourth exon region of sheep IGFBP-3, and two alleles A and B (alle frequency:56.76% and 43.24%), showing three genotypes of AA, AB and BB (genotype frequency:29.73%, 54.05%, 16.22%), and the population studied was in the Hardy-Weinberg equilibrium state at this locus (P>0.05).The correlation results showed that the mutation at g.87 A>C of Gansu alpine merino sheep IGFBP-3 gene had a very significant effect on live weight and hot carcass weight before slaughter (P<0.01);it had a significant effect on eye muscle area, GR value and tenderness.Degree had a significant impact (P<0.05).It showed that the sheep population of allele B had a higher slaughter rate, and it could be used as a molecular marker for the selection of slaughter traits of Gansu alpine merino sheep in production practice.

Key words: Gansu alpine merino sheep, IGFBP-3 gene, Variation, Meat quality traits

CLC Number:

Q78
S826

SHI Fengyun, ZHU Guangqin, LIAO Yunqiong, WANG Bing. Correlation Analysis of IGFBP-3 Gene Nucleotide Variation and Ovine Slaughter Traits[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(S1): 368-372. doi: 10.7668/hbnxb.20191949.

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