Variation of FGF18 Gene in Goats and Its Effect on Cashmere Traits

doi:10.7668/hbnxb.20191285

Abstract

Abstract: In order to study the polymorphism of FGF18 gene in goats and its effect on some cashmere traits, PCR-SSCP combined sequencing method were used to analyze three regions(Exon 3, Exon 4 and Exon 5) of FGF18 gene in three goat breeds(Longdong cashmere goat, Chaidamu cashmere goat, and Zhongwei goat), then the association between gene polymorphism and variation of Longdong cashmere goat traits was analyzed. The results showed that FGF18 gene was conserved among the three goat breeds, and only one synonymous mutation was found in Exon 5(c.498T>C). Three genotypes EE, EF and FF caused by c.498T>C locus were detected in all three goat breeds, and the distribution in the three goat breeds was at Hardy-Weinberg equilibrium with respect to this SNP. Allele E was the dominant allele in all three goat breeds; the dominant genotypes of Longdong cashmere goats, Chaidamu cashmere goats and Zhongwei goats were EF, EE and EF, respectively. The cashmere yield and height of Longdong cashmere goats with FF genotype goats were significantly higher than those goats with EE genotype and EF genotype(P<0.05). The results showed that the FGF18 gene was moderately polymorphic in the three goat populations and the c.498T>C site was significantly associated with the cashmere yield and the height of down layer of the Longdong cashmere goats. It was suggested that FGF18 gene can be used as a candidate gene for molecular improvement when these two traits are used as the breeding targets.

Key words: Goat, FGF18, Gene variation, Cashmere yield, Height of down layer

CLC Number:

Q78
S826

ZHANG Ruiguo, LI Shaobin, WANG Jiqing, LIU Xiu, LUO Yuzhu. Variation of FGF18 Gene in Goats and Its Effect on Cashmere Traits[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(6): 202-207. doi: 10.7668/hbnxb.20191285.

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