Study on Differentially Expressed Genes Related to Fat Deposition Gene in Angus and Chinese Simmental Cattle Base on Transcriptome

doi:10.7668/hbnxb.20190684

Abstract

Abstract: To identify differentially expressed genes related to fat deposition in Angus and Chinese simmental cattle and analyze differentially expressed gene functions and pathways, the total RNA was extracted from the backfat, then sequenced by BGISEQ-500. The differentially expressed genes were screened by bioinformatics analysis, GO and KEGG enrichment analysis were used to study the function of differentially expressed genes and the candidate genes related to fat deposition were screened. The accuracy of RNA-seq results was verified by qRT-PCR. The results showed that there were 1 296 significant differentially expressed genes in Angus cattle compared with Chinese simental cattle, including 802 up genes and 494 down genes. Differential transcripts of GO function enrichment analysis revealed that molecular functions, cell components, and biological processes were enriched to 890 entries, 3 149 entries,and 2 205 entries. KEGG analysis showed that the differential transcripts were significantly enriched in 6 pathways. The cAMP pathway associated with fat deposition was analyzed, and the differentially expressed genes associated with fat deposition were screened out, the genes DKKL1, ACACB and PTGS2 were verified by qRT-PCR, and they were preliminarily identified as candidate genes for fat deposition. At the same time, a large number of differentially expressed genes were obtained by sequencing the backfat tissues of Chnese simmental cattle and Angus cattle, which laid a foundation for the subsequent screening of genes related to fat deposition.

Key words: Cattle, Fat deposition, DKKL1, ACACB, PTGS2, qRT-PCR

CLC Number:

Q78
S832

HAN Xinrong, WU Huiguang, WU Jianghong, WANG Guofu, GAO Shuxin. Study on Differentially Expressed Genes Related to Fat Deposition Gene in Angus and Chinese Simmental Cattle Base on Transcriptome[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(6): 208-216. doi: 10.7668/hbnxb.20190684.

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